FastQCFastQC Report
Thu 26 May 2016
SRR1512802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810813
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43540.24044448543278626No Hit
GTCCTACAGTGGACATTTCTAAATT43240.23878777101776935No Hit
CTGTAGGACGTGGAATATGGCAAGA39660.21901764566523435No Hit
GTCCTAAAGTGTGTATTTCTCATTT38050.21012661163797697No Hit
CTTTAGGACGTGAAATATGGCGAGG33680.1859938049925641No Hit
GGTATCAACGCAGAGTACTTTTTTT26370.14562519707998564No Hit
GTCCTACAGTGTGCATTTCTCATTT25270.13955057755825698No Hit
TATCAACGCAGAGTACTTTTTTTTT23200.12811924809464037No Hit
GTACATGGGAAGCAGTGGTATCAAC20580.11365060887015943No Hit
CTGTAGGACCTGGAATATGGCGAGA18800.10382077000772581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8450.013.1484214
TATGTCG1157.0584065E-811.56719816
GGCGAGG10200.011.45020219
AATGTCC6000.011.0382688
AGGACCT15850.011.0235365
TTTAGAA7100.010.968222
AAATGTC5950.010.8526657
TTGGACT1855.456968E-1210.7793354
TGTCGAG1252.2572931E-710.63413318
GTCCTAC23250.010.5546831
TAGACTG2001.8189894E-1210.4453995
GTCCTAA11350.010.4288691
GGACCTG15850.010.424726
TGGCGAG22600.010.41903118
GCCTAAC650.00967298910.3636481
GTGTAGG3650.010.2825241
TCCTACA26600.010.2466262
TTAGGAC15000.010.1299983
GGACGTA750.002656995610.1291536
CATTTCT31200.010.0785714