FastQCFastQC Report
Thu 26 May 2016
SRR1512802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810813
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53480.29533695638367957No Hit
GTCCTACAGTGGACATTTCTAAATT47020.25966237264698233No Hit
CTGTAGGACGTGGAATATGGCAAGA45490.2512131291303961No Hit
GTCCTAAAGTGTGTATTTCTCATTT40580.22409823653795286No Hit
CTTTAGGACGTGAAATATGGCGAGG39330.21719525980871573No Hit
GGTATCAACGCAGAGTACTTTTTTT34400.18996991958860468No Hit
TATCAACGCAGAGTACTTTTTTTTT31530.17412068501827632No Hit
GTCCTACAGTGTGCATTTCTCATTT27990.15457145492107688No Hit
GTACATGGGAAGCAGTGGTATCAAC23380.1291132767436505No Hit
CTGTAGGACCTGGAATATGGCGAGA21660.11961478076422027No Hit
CTGAAGGACCTGGAATATGGCGAGA20560.11354016124249164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7650.013.6701324
AATCCCG905.447564E-712.65878719
GGCGAGG10900.012.19424419
AGGACCT14250.011.8092925
CGTCCAA600.005881766311.08194710
GGACCTG14950.011.0554736
GTCCTAA11600.010.9107761
TGTAGGA26150.010.9072442
AAATGTC5350.010.8304217
GGACGTG24550.010.7562916
TTAGGAC14550.010.7157433
AGGACGT25550.010.6796365
GTAGGAC25200.010.5632833
GACGTGA13050.010.5542357
ATTTAGA6350.010.4902611
TAGAAAT6300.010.4123794
CTGTAGG25700.010.4048351
CAGGACT3200.010.3982324
ACCTTTT5300.010.3906215
TGGCGAG24100.010.3607718
Produced by FastQC (version 0.11.5)