FastQCFastQC Report
Thu 26 May 2016
SRR1512801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019082
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52490.2599696297624366No Hit
GGTATCAACGCAGAGTACTTTTTTT30560.15135591323185488No Hit
TATCAACGCAGAGTACTTTTTTTTT28830.14278766290819295No Hit
GTCCTACAGTGGACATTTCTAAATT25850.12802848026974634No Hit
GTCCTAAAGTGTGTATTTCTCATTT23920.11846968077571887No Hit
CTGTAGGACGTGGAATATGGCAAGA23170.11475512138684808No Hit
CTTTAGGACGTGAAATATGGCGAGG20930.10366097067875402No Hit
GTACATGGGAAGCAGTGGTATCAAC20290.10049121333358428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5100.013.0319514
CGAAATC853.956342E-612.29094113
TGACCGC600.00590000611.0774346
ACCGTGC1201.3430326E-711.0422248
ACGAAAT1053.4925633E-610.85055312
GTATAAG2300.010.4570891
TGTAGGA16550.010.4415692
GTAAGAC1659.385985E-1010.3581193
GTAGGAC15650.010.3139953
AGGACCT12450.010.29546455
GTATTAT3300.010.2035851
GGACCTG12100.010.2011796
GTCCTAG2650.010.1650821
GGACGTG14600.09.8201126
GGACTGT3000.09.8114416
TAGGACT3600.09.7584494
AGGACGT15500.09.6784765
GTTTTAG1502.2050153E-79.6205231
ATCCTAC2201.2732926E-119.62052251
TTAGGAC8700.09.604083
Produced by FastQC (version 0.11.5)