FastQCFastQC Report
Thu 26 May 2016
SRR1512801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019082
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60980.3020184420444538No Hit
GGTATCAACGCAGAGTACTTTTTTT38620.19127504479758622No Hit
TATCAACGCAGAGTACTTTTTTTTT36450.18052758629912008No Hit
GTCCTACAGTGGACATTTCTAAATT29070.14397632191263157No Hit
CTGTAGGACGTGGAATATGGCAAGA27560.13649767567637175No Hit
GTCCTAAAGTGTGTATTTCTCATTT24580.12173849303792515No Hit
CTTTAGGACGTGAAATATGGCGAGG24320.12045077911644994No Hit
GTACATGGGAAGCAGTGGTATCAAC22360.11074339724686764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGACG550.003051495412.1001425
TTCTACG550.003052062412.0998422
AAACCGT550.003072530712.0890486
TAGGACC6350.011.9776444
GTCCTAT1657.2759576E-1211.5345291
CTTAGGC1850.011.3059233
GGACCGG600.005883422711.0816286
TGTAGGA16050.010.8397792
GTAGGAC15750.010.7447073
TACACCG803.7452136E-410.6956615
ACCGTAT909.537033E-510.5534098
AGTGCGG1002.4047391E-510.44787510
ACCGTGC1201.5280548E-610.2895748
CTGTAGG16600.010.203891
GGCGTCA750.002652084210.13152312
GACGTGG7800.010.1069177
CTAGACA2550.010.0665054
TGGACCG951.6317567E-410.0076365
TAGGACA5150.09.9687724
GTACTAA1151.0063119E-59.9297261
Produced by FastQC (version 0.11.5)