FastQCFastQC Report
Thu 26 May 2016
SRR1512800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990899
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48550.24385968348971998No Hit
GGTATCAACGCAGAGTACTTTTTTT29030.1458135244429778No Hit
GTACATGGGAAGCAGTGGTATCAAC28740.14435689605550056No Hit
TATCAACGCAGAGTACTTTTTTTTT25660.12888649800919083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1302.1518645E-911.8386631
GTATAGG2500.011.5426971
CCTACAC2200.011.2243593
TAGGACC2900.010.806724
TAAGCCC803.7833618E-410.6839154
GTCCTAG2200.010.493361
TCCAACG1553.7016434E-910.41270118
CTATTAG951.4502561E-410.1251731
GTATAAG2300.010.0371281
TAAGGCA2002.5465852E-119.9716554
TATACTG3550.09.89808755
AGGACGT8350.09.8948845
GTAGGAC8050.09.7924883
GTCCTAA5250.09.7105231
CTAGAGT2350.09.6988744
TGTAGGA9300.09.5996582
GAGAGTC1901.0586518E-99.4968137
CCAACGA1106.8718466E-59.49561319
CCGTGCC1502.7375063E-79.4783599
GGACTGT2950.09.3363246
Produced by FastQC (version 0.11.5)