FastQCFastQC Report
Thu 26 May 2016
SRR1512800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990899
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60120.3019741332935523No Hit
GGTATCAACGCAGAGTACTTTTTTT35880.1802200915264913No Hit
TATCAACGCAGAGTACTTTTTTTTT34110.17132963550637173No Hit
GTACATGGGAAGCAGTGGTATCAAC32030.16088209396860415No Hit
CCCATGTACTCTGCGTTGATACCAC22680.11391838561373531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG2150.011.5078761
TAGGACC4200.010.8651794
ACACCGT1252.2442873E-710.6387756
GTAATAC1451.4277248E-810.4902533
GGATATA1701.5133992E-910.0759011
GTCCTAC8750.09.8967731
GTAGGAC10050.09.64868453
TGTAGGA10000.09.507752
TAGAACA3900.09.5070314
TAAGACT2550.09.320624
TAGGACT3100.09.2003534
CCGTCCA1252.7587641E-59.1187219
CAGGACA6500.09.0682464
CAGACCG1054.4991597E-49.04635059
TCCTACA9400.09.0020182
TACACCG850.0073851948.9486945
GAGCGCG850.00744331268.94014711
GTGTAAT2252.5283953E-108.8817211
TAGGTTG1502.6710022E-68.8741225
GACGTGG4950.08.8270457
Produced by FastQC (version 0.11.5)