FastQCFastQC Report
Thu 26 May 2016
SRR1512799_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512799_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911360
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45140.23616691779675203No Hit
GTCCTACAGTGGACATTTCTAAATT36220.18949857692951616No Hit
GTCCTAAAGTGTGTATTTCTCATTT33440.17495395948434622No Hit
CTGTAGGACGTGGAATATGGCAAGA31130.16286832412523022No Hit
GGTATCAACGCAGAGTACTTTTTTT26880.14063284781516827No Hit
CTTTAGGACGTGAAATATGGCGAGG26850.14047589151180312No Hit
TATCAACGCAGAGTACTTTTTTTTT23610.12352461074836767No Hit
GTCCTACAGTGTGCATTTCTCATTT22370.1170370835426084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG250.006049632618.9907055
AACAGCG400.005296838514.243037
CCAACGA1001.0160875E-813.29209319
TAGGACC6600.013.2359474
TACACCG604.1096972E-412.660475
GGCGAGG8900.012.16130619
CGTGCGC600.00591527211.07353910
GTATTAA2800.010.9921981
GTCCTAC19150.010.8989221
TTTAGAA6200.010.874572
AGGCGTG1752.0008883E-1110.8518317
GGACCTG13450.010.8011216
AGGACCT14100.010.7748695
GTAGGAC21000.010.671813
TGGCGAG21100.010.6173218
AGTACCG909.564028E-510.5503925
TCCAACG1803.6379788E-1110.54733818
TCCTACA21750.010.52211952
TCAGGAC2800.010.5135433
GGCCTAT1201.3002664E-610.4196861
Produced by FastQC (version 0.11.5)