FastQCFastQC Report
Thu 26 May 2016
SRR1512799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911360
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54330.2842478653942742No Hit
GTCCTACAGTGGACATTTCTAAATT38170.19970073664825047No Hit
CTGTAGGACGTGGAATATGGCAAGA36030.18850452034153692No Hit
GTCCTAAAGTGTGTATTTCTCATTT33240.17390758412857862No Hit
GGTATCAACGCAGAGTACTTTTTTT32360.16930353256320108No Hit
CTTTAGGACGTGAAATATGGCGAGG32030.16757701322618448No Hit
TATCAACGCAGAGTACTTTTTTTTT30300.15852586639879457No Hit
GTCCTACAGTGTGCATTTCTCATTT24160.1264021429767286No Hit
GTACATGGGAAGCAGTGGTATCAAC19720.10317261007868743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA751.4806583E-512.6652887
TCCAACG1251.4242687E-912.15263118
GTCGAGG1103.8477083E-812.08358319
TAGGACC7400.011.81973654
AGGCGTG1459.822543E-1111.7918217
GTGCGCC907.472003E-611.60984711
GTCTATA1751.8189894E-1211.417591
ATGTCGA1553.1650416E-1011.02557317
AAGGCGT1408.363713E-910.8562456
GTATTAG1605.293259E-1010.703991
CGTGCGC909.5272844E-510.55440610
TGTCGAG1355.7392754E-810.5491618
GTAGGAC21750.010.5343923
AATCCCG1909.094947E-1210.49363819
GTCCTAG3550.010.4527231
GACGTGG10000.010.3538727
TGTAGGA22750.010.3226212
TGCGCCA1201.5265541E-610.290277512
TAGGACA6700.010.216674
GTATTAC2053.6379788E-1210.2108531
Produced by FastQC (version 0.11.5)