FastQCFastQC Report
Thu 26 May 2016
SRR1512798_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512798_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129971
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48710.22868855960949705No Hit
GTCCTACAGTGGACATTTCTAAATT31670.14868747039278937No Hit
GGTATCAACGCAGAGTACTTTTTTT29300.13756055833624026No Hit
CTGTAGGACGTGGAATATGGCAAGA27700.1300487189731691No Hit
GTCCTAAAGTGTGTATTTCTCATTT27480.12901584106074684No Hit
TATCAACGCAGAGTACTTTTTTTTT26760.1256355133473648No Hit
CTTTAGGACGTGAAATATGGCGAGG23060.10826438482026281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGT604.1149795E-412.65866856
ACCGTCC604.217738E-412.6217068
TAGGACC7750.012.127824
TACGACT550.003083629812.0832734
GTCCTAC15800.011.3858121
GCGATAC855.319276E-511.17759714
CTAGGAC2550.011.1699413
TCCAACG1451.2332748E-911.1293218
TGTAGGA21450.011.1100552
GAATAGC600.005904542311.0763356
ACGACTG600.005904542311.0763355
AGGACCT12700.010.989125
GTAGGAC20750.010.9357763
TCCTACA17850.010.903992
GGACGTG17450.010.7725636
GGACGCT803.7931398E-410.6810047
GACGTGG9250.010.5719487
AGGACGT17800.010.5607425
TAGGACT3550.010.430034
TAGGACA6250.010.32947354
Produced by FastQC (version 0.11.5)