FastQCFastQC Report
Thu 26 May 2016
SRR1512798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129971
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58420.27427603474413503No Hit
GGTATCAACGCAGAGTACTTTTTTT36300.17042485554967648No Hit
GTCCTACAGTGGACATTTCTAAATT34920.16394589409902766No Hit
TATCAACGCAGAGTACTTTTTTTTT33770.15854675955682024No Hit
CTGTAGGACGTGGAATATGGCAAGA33030.15507253385139985No Hit
GTCCTAAAGTGTGTATTTCTCATTT29540.13868733424070093No Hit
CTTTAGGACGTGAAATATGGCGAGG26980.1266683912597871No Hit
GTACATGGGAAGCAGTGGTATCAAC22810.10709065991978295No Hit
GTCCTACAGTGTGCATTTCTCATTT22150.10399202618251609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG550.003069058812.09092410
TACCGGC657.975003E-411.7011182
TAGGACC6350.011.6767134
GGACGTG16200.011.3173846
AGGACGT16600.011.1675235
TTAGGAC10500.011.1356493
TGTAGGA19050.011.0792092
GTGCGCC1053.4675213E-610.85715611
TAGGACG17350.010.8483934
GTATTAG1951.8189894E-1210.7346071
GTAGGAC18250.010.678023
CCGTCCA909.5178286E-510.5555699
AAGGCGT1355.6938006E-810.5550746
TTTAGGA11400.010.5079132
GACGTGA8600.010.4941997
ATTAGAC1451.4297257E-810.4894263
CTGTAGG18800.010.3751431
TCCAACG1106.0984166E-610.3563518
GTCCTAC18300.010.3466571
GACGTGG8950.010.2961027
Produced by FastQC (version 0.11.5)