FastQCFastQC Report
Thu 26 May 2016
SRR1512797_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512797_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977569
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50180.25374588699559913No Hit
GTCCTAAAGTGTGTATTTCTCATTT46150.23336733130424273No Hit
CTGTAGGACGTGGAATATGGCAAGA44250.22375957551923598No Hit
GTATCAACGCAGAGTACTTTTTTTT44190.22345617270497264No Hit
CTTTAGGACGTGAAATATGGCGAGG38460.1944812039428207No Hit
GTACATGGGAAGCAGTGGTATCAAC31190.15771889628124228No Hit
GTCCTACAGTGTGCATTTCTCATTT30400.15372409256010788No Hit
GGTATCAACGCAGAGTACTTTTTTT26480.13390177536156767No Hit
TATCAACGCAGAGTACTTTTTTTTT23860.12065318580540046No Hit
CCCATGTACTCTGCGTTGATACCAC21430.10836537182773395No Hit
CTGTAGGACCTGGAATATGGCGAGA20240.1023478826781771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC1251.03682396E-1012.9147043
GTTCCGT751.4872439E-512.6598646
CCAACGA1501.8189894E-1212.65889919
TCCAACG2350.012.52231718
TAGGACC9550.012.3284544
CGGTTTC1551.8189894E-1212.25366413
CCAACGT1103.848436E-812.08349319
ACCGTGC1002.027029E-611.3537228
GATATAC5450.011.1223511
CCGTGCG600.005972023611.0593729
GGCGAGG12250.010.85048419
TAGACAG3250.010.8095775
GTGTAGG3750.010.7763211
ACCTTTT6550.010.73416215
TGGCGAG25550.010.6630618
GTCCTAA14100.010.6453031
TGTAGGA28400.010.4992162
GTCCTAT3500.010.4464351
ATAGGAC2300.010.3218563
CCGGTTT1752.382876E-1010.30664912
Produced by FastQC (version 0.11.5)