##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512797_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1977569 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.90356290981503 31.0 25.0 34.0 10.0 34.0 2 28.43937126846143 31.0 28.0 34.0 16.0 34.0 3 29.598986938003176 31.0 30.0 34.0 23.0 34.0 4 33.428638393906866 35.0 35.0 37.0 28.0 37.0 5 33.792902295697395 37.0 35.0 37.0 28.0 37.0 6 34.07059627249416 37.0 35.0 37.0 30.0 37.0 7 34.052965534957316 37.0 35.0 37.0 30.0 37.0 8 34.09082211543567 37.0 35.0 37.0 30.0 37.0 9 35.68377993384807 39.0 37.0 39.0 30.0 39.0 10 35.61507689491492 39.0 37.0 39.0 30.0 39.0 11 35.65026959868404 39.0 37.0 39.0 30.0 39.0 12 35.563341658369445 39.0 37.0 39.0 30.0 39.0 13 35.556847826801494 39.0 37.0 39.0 30.0 39.0 14 36.757084076459535 40.0 38.0 41.0 30.0 41.0 15 36.67354059453804 40.0 38.0 41.0 30.0 41.0 16 36.586541860233446 40.0 38.0 41.0 30.0 41.0 17 36.54910650399556 40.0 38.0 41.0 30.0 41.0 18 36.477989895674945 40.0 37.0 41.0 30.0 41.0 19 36.499534023844426 40.0 38.0 41.0 29.0 41.0 20 36.42986414127649 40.0 37.0 41.0 29.0 41.0 21 36.33128856692232 40.0 37.0 41.0 29.0 41.0 22 36.2073394152113 40.0 37.0 41.0 28.0 41.0 23 36.06842441401539 40.0 37.0 41.0 27.0 41.0 24 35.96302531036844 40.0 37.0 41.0 27.0 41.0 25 35.85460836006228 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.5279043906005647 1101 2 2.2108172022968873 1101 3 1.1539132795022269 1101 4 0.9040588964774159 1101 5 0.5641693481347687 1101 6 0.5169083295619075 1101 7 0.1555133245617526 1101 8 0.20503760939787696 1101 9 0.15180976983190675 1101 10 -0.08964474068540795 1101 11 -0.04321453823682475 1101 12 0.04342742296729085 1101 13 0.04794377300517283 1101 14 -0.15157250764595886 1101 15 -0.23196053380361548 1101 16 -0.19403203112727851 1101 17 -0.45231463413670525 1101 18 -0.18936638772972714 1101 19 -0.1875829743915034 1101 20 -0.09784727046245223 1101 21 -0.13645470330909149 1101 22 -0.25264428434032027 1101 23 -0.3220523310423147 1101 24 -0.3334529541060931 1101 25 -0.39288852358836124 1102 1 3.5465948555879656 1102 2 2.1734205880158015 1102 3 1.0160900520902416 1102 4 0.872528212746225 1102 5 0.5269361120441332 1102 6 0.30721830373508396 1102 7 -0.0341556842946531 1102 8 0.13421120329982017 1102 9 0.011688791549438804 1102 10 -0.17232978935903986 1102 11 -0.08132086737722233 1102 12 -0.2584183584771296 1102 13 -0.1436570195751088 1102 14 -0.21819551966013506 1102 15 -0.3566350844482429 1102 16 -0.47152662004160817 1102 17 -0.25521387815314256 1102 18 -0.3550710836110298 1102 19 -0.49928764936039727 1102 20 -0.43930115337387576 1102 21 -0.43502899986795285 1102 22 -0.2718889281497283 1102 23 -0.5140805209688111 1102 24 -0.4685745597608033 1102 25 -0.5395472435398432 1103 1 2.1152073774565565 1103 2 0.7720059643374668 1103 3 -0.1373671557859666 1103 4 -0.7128653855602991 1103 5 -0.9067534030338322 1103 6 -1.0628722501237888 1103 7 -1.113988325147787 1103 8 -1.2152137572670014 1103 9 -1.511437359469035 1103 10 -1.4588538257163748 1103 11 -1.452420134726303 1103 12 -1.3154415867725149 1103 13 -1.4018343276437264 1103 14 -1.7650852389055913 1103 15 -1.9008308597330625 1103 16 -2.0602042960724205 1103 17 -1.8979250138893633 1103 18 -2.0717392859793264 1103 19 -2.0554279502528416 1103 20 -1.8717958521722764 1103 21 -2.1211760416822827 1103 22 -2.313695677073426 1103 23 -2.377112505188265 1103 24 -2.3309454850493267 1103 25 -2.4866855645895853 1104 1 -6.932045387848138 1104 2 -8.349614761668661 1104 3 -7.817321691893962 1104 4 -6.364881663287697 1104 5 -4.3155633491240515 1104 6 -2.991922578187747 1104 7 -2.44478260641781 1104 8 -2.1792909949022174 1104 9 -2.204382185926228 1104 10 -2.195532582477753 1104 11 -1.87740952780657 1104 12 -2.132199613619207 1104 13 -2.0782555840808072 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0.26962718410810993 2314 21 0.12875419715250302 2314 22 -0.20944390559727566 2314 23 0.013485405178933263 2314 24 0.033168342299795484 2314 25 0.13876216485602555 2315 1 -0.2547769214137716 2315 2 1.0266004567950766 2315 3 0.7995448953934172 2315 4 0.6528719601839725 2315 5 0.6601158636919209 2315 6 0.3382916129839515 2315 7 0.3606041877977688 2315 8 0.29866757098558594 2315 9 -0.43141333539096394 2315 10 -0.07685298892263859 2315 11 -0.04869640262626973 2315 12 0.029445648523982015 2315 13 0.11848491366329483 2315 14 0.15840437090559334 2315 15 0.18338163188774814 2315 16 0.17368629989567097 2315 17 0.03450164293973046 2315 18 0.11635259937093423 2315 19 0.05380512379440461 2315 20 0.0348634063205111 2315 21 0.05504327025732891 2315 22 -0.532548729795927 2315 23 -0.10889762015742122 2315 24 0.12510259909792865 2315 25 0.21033436999653787 2316 1 0.9222029599874979 2316 2 0.9705307537665284 2316 3 0.5440955160430718 2316 4 0.3341199407483657 2316 5 0.14907706471074533 2316 6 -0.1533868128068221 2316 7 -0.1305316796996152 2316 8 -0.19440617234847934 2316 9 -0.1106203005824824 2316 10 -0.20837715134746304 2316 11 -0.13714201027720918 2316 12 -0.23719065435002307 2316 13 -0.20594747335066899 2316 14 -0.1412871753816347 2316 15 -0.12324803667576134 2316 16 -0.08079987875451877 2316 17 -0.034258134779577176 2316 18 0.016703247715710745 2316 19 0.014056633008770802 2316 20 -0.08679521824402059 2316 21 -0.03673018472937173 2316 22 -0.0463228779428988 2316 23 0.04358590424700992 2316 24 -0.057524008920459835 2316 25 0.0949376583092203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69477.0 3 2.0 4 630.0 5 2526.0 6 3021.0 7 2871.0 8 2561.0 9 2459.0 10 2520.0 11 2500.0 12 2523.0 13 2633.0 14 2619.0 15 2516.0 16 2618.0 17 2753.0 18 3274.0 19 3973.0 20 5009.0 21 5739.0 22 6674.0 23 7886.0 24 9321.0 25 10703.0 26 12376.0 27 14045.0 28 16404.0 29 19807.0 30 24853.0 31 32694.0 32 43679.0 33 59944.0 34 87816.0 35 131048.0 36 234742.0 37 329336.0 38 814017.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.528755511895504 20.861247663120974 11.737320646303678 23.872676178679843 2 14.166228770488976 20.10567330848653 42.511620310537715 23.216477610486784 3 18.812985378238118 24.90314623232631 29.499180185283702 26.78468820415187 4 11.919496322022827 16.984626745162636 38.915605334588406 32.18027159822613 5 12.76206503392086 38.172077368779824 36.15351218878826 12.912345408511053 6 31.457818008778315 37.27181922807359 18.447947595641956 12.822415167506135 7 27.861674146706346 31.461849765029186 22.567310193993205 18.109165894271264 8 24.513452393082712 37.88113592916639 20.81502649010987 16.790385187641025 9 26.682131866082255 15.11338893641867 20.532459686411574 37.6720195110875 10 15.027340264296823 28.842851373448603 35.039471223934775 21.090337138319796 11 33.7621869710615 22.112930444096698 25.620152268387354 18.504730316454445 12 23.89736820227066 24.48358649609357 32.289201700864425 19.32984360077135 13 29.230181902802993 22.195548935397863 25.74751815910044 22.826751002698707 14 20.501235289106333 22.878203333860714 27.817838467186746 28.802722909846207 15 22.626725933211837 32.01775354458683 23.238934645068824 22.116585877132504 16 21.366478138170486 28.03922735356747 29.19323331710981 21.40106119115223 17 21.453268720558224 28.994177628852192 28.414991804741806 21.137561845847777 18 21.23596029693142 27.217479652126652 31.201931412938883 20.344628638003044 19 23.62506899045613 26.641136607945164 27.762367727113542 21.971426674485162 20 23.548490578181354 27.516636352717537 28.63616622213528 20.298706846965825 21 22.46228586039376 26.78799722394711 27.511333520026948 23.23838339563218 22 22.99243237318402 28.74307772485057 27.386659399958695 20.877830502006717 23 21.65582574875487 28.43266946681379 28.30861360204992 21.602891182381427 24 23.123667301659474 27.00695109066865 29.04229417454149 20.827087433130387 25 22.36280066914619 27.636539523476962 28.46424250052785 21.536417306849003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1851.0 1 1851.0 2 1597.5 3 1344.0 4 1344.0 5 1344.0 6 2199.0 7 3054.0 8 3054.0 9 3054.0 10 3926.5 11 4799.0 12 4799.0 13 4799.0 14 7450.0 15 10101.0 16 10101.0 17 10101.0 18 17523.5 19 24946.0 20 24946.0 21 24946.0 22 39286.0 23 53626.0 24 53626.0 25 53626.0 26 77745.0 27 101864.0 28 101864.0 29 101864.0 30 132423.0 31 162982.0 32 162982.0 33 162982.0 34 181537.5 35 200093.0 36 200093.0 37 200093.0 38 212652.0 39 225211.0 40 225211.0 41 225211.0 42 235035.5 43 244860.0 44 244860.0 45 244860.0 46 252729.5 47 260599.0 48 260599.0 49 260599.0 50 246614.5 51 232630.0 52 232630.0 53 232630.0 54 203266.5 55 173903.0 56 173903.0 57 173903.0 58 148791.0 59 123679.0 60 123679.0 61 123679.0 62 100722.0 63 77765.0 64 77765.0 65 77765.0 66 59999.0 67 42233.0 68 42233.0 69 42233.0 70 30920.0 71 19607.0 72 19607.0 73 19607.0 74 13642.0 75 7677.0 76 7677.0 77 7677.0 78 5326.5 79 2976.0 80 2976.0 81 2976.0 82 1993.5 83 1011.0 84 1011.0 85 1011.0 86 709.0 87 407.0 88 407.0 89 407.0 90 293.5 91 180.0 92 180.0 93 180.0 94 144.5 95 109.0 96 109.0 97 109.0 98 85.5 99 62.0 100 62.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7330368750723741 2 0.3044647241132926 3 0.29237917867846835 4 0.294958102599707 5 0.2994080105422364 6 0.21733754928399462 7 0.4887313666425798 8 0.2501050532244387 9 0.2294230947188189 10 0.2076286592275668 11 0.24616081663901487 12 0.2490431433745169 13 0.2600162118237088 14 0.2812038416864342 15 0.46956642220827693 16 0.5708018279008217 17 0.5657451143297655 18 0.2592577047880504 19 0.22603509662621127 20 0.21895569762673261 21 0.3248938469403596 22 0.3508347875598778 23 0.460211502101823 24 0.3510876232384306 25 0.3689883892799695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1977569.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.38849545058397 #Duplication Level Percentage of deduplicated Percentage of total 1 86.61120078137043 63.562657145132185 2 9.37240460859588 13.75653345957942 3 2.093644589541552 4.609482793041298 4 0.7404600698431476 2.173650018680917 5 0.34750801344449994 1.2751545131856579 6 0.20201049904076349 0.8895147953893962 7 0.1334330107134823 0.6854713529792883 8 0.08770959231205236 0.5149500013092503 9 0.06231271940550993 0.41157330521443025 >10 0.30650744733340285 3.9605419670923507 >50 0.020834855255646555 1.063178836034772 >100 0.017455408819203246 2.6787110260703537 >500 0.002711042594686015 1.4728602620968825 >1k 0.001737847817106408 2.6897261770910483 >5k 6.951391268425632E-5 0.25599434710270236 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5018 0.25374588699559913 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4615 0.23336733130424273 No Hit CTGTAGGACGTGGAATATGGCAAGA 4425 0.22375957551923598 No Hit GTATCAACGCAGAGTACTTTTTTTT 4419 0.22345617270497264 No Hit CTTTAGGACGTGAAATATGGCGAGG 3846 0.1944812039428207 No Hit GTACATGGGAAGCAGTGGTATCAAC 3119 0.15771889628124228 No Hit GTCCTACAGTGTGCATTTCTCATTT 3040 0.15372409256010788 No Hit GGTATCAACGCAGAGTACTTTTTTT 2648 0.13390177536156767 No Hit TATCAACGCAGAGTACTTTTTTTTT 2386 0.12065318580540046 No Hit CCCATGTACTCTGCGTTGATACCAC 2143 0.10836537182773395 No Hit CTGTAGGACCTGGAATATGGCGAGA 2024 0.1023478826781771 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 5.0567135710561806E-5 0.0 11 0.0 0.0 0.0 5.0567135710561806E-5 0.0 12 0.0 0.0 0.0 5.0567135710561806E-5 0.0 13 0.0 0.0 0.0 5.0567135710561806E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAATAC 125 1.03682396E-10 12.914704 3 GTTCCGT 75 1.4872439E-5 12.659864 6 CCAACGA 150 1.8189894E-12 12.658899 19 TCCAACG 235 0.0 12.522317 18 TAGGACC 955 0.0 12.328454 4 CGGTTTC 155 1.8189894E-12 12.253664 13 CCAACGT 110 3.848436E-8 12.083493 19 ACCGTGC 100 2.027029E-6 11.353722 8 GATATAC 545 0.0 11.122351 1 CCGTGCG 60 0.0059720236 11.059372 9 GGCGAGG 1225 0.0 10.850484 19 TAGACAG 325 0.0 10.809577 5 GTGTAGG 375 0.0 10.776321 1 ACCTTTT 655 0.0 10.734162 15 TGGCGAG 2555 0.0 10.66306 18 GTCCTAA 1410 0.0 10.645303 1 TGTAGGA 2840 0.0 10.499216 2 GTCCTAT 350 0.0 10.446435 1 ATAGGAC 230 0.0 10.321856 3 CCGGTTT 175 2.382876E-10 10.306649 12 >>END_MODULE