FastQCFastQC Report
Thu 26 May 2016
SRR1512797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977569
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57060.28853607636446565No Hit
GTCCTACAGTGGACATTTCTAAATT53940.2727591300227704No Hit
CTGTAGGACGTGGAATATGGCAAGA51280.25930827192376094No Hit
GTCCTAAAGTGTGTATTTCTCATTT48210.24378416126061847No Hit
CTTTAGGACGTGAAATATGGCGAGG44480.2249226196405789No Hit
GTACATGGGAAGCAGTGGTATCAAC35960.18183942001518025No Hit
GGTATCAACGCAGAGTACTTTTTTT35670.18037297307957398No Hit
GTCCTACAGTGTGCATTTCTCATTT34470.17430491679430654No Hit
TATCAACGCAGAGTACTTTTTTTTT33630.17005727739461937No Hit
CTGTAGGACCTGGAATATGGCGAGA24140.12206906560529619No Hit
CCCATGTACTCTGCGTTGATACCAC23960.12115885716250609No Hit
CTGAAGGACCTGGAATATGGCGAGA23160.11711348630566115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTGA707.2769326E-613.56926110
TAGGACC11250.013.0987274
CTACGAC701.08356195E-412.2234673
AGGACCG951.030523E-612.0093245
GGACCGT951.0419262E-611.9983866
ATAGGAC3050.011.5332533
GGCGAGG11550.011.50836219
TGGACCG752.0594469E-411.4088585
TCCAACG1751.8189894E-1211.39327918
TTTGCGT600.00590246911.07679919
GTCGAGG1303.295645E-810.95507519
AGGACCT20200.010.8252365
GACCGTG1158.8373963E-710.7374157
GTCCTAC27150.010.7247361
GTATTAG2050.010.676011
TTAGGAC16900.010.6322463
TGGCGAG27600.010.62959918
TGTAGGA29400.010.5748052
TGTCGAG1355.738366E-810.54933218
GACGTGA14850.010.4899067
Produced by FastQC (version 0.11.5)