FastQCFastQC Report
Thu 26 May 2016
SRR1512796_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512796_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139487
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46640.217996183197187No Hit
GGTATCAACGCAGAGTACTTTTTTT28560.13348994408472684No Hit
TATCAACGCAGAGTACTTTTTTTTT25900.12105705713565916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6050.013.969554
GTAGGAC13900.011.2729443
TTAGGAC8700.011.2431033
ATACCGT855.3467644E-511.17194756
ACCGTCC600.00605852211.0380898
AGGACGT11950.010.9662715
GTGTAGG4500.010.9002311
GGACGTG11650.010.8411036
GGCGAGG5800.010.8041919
AAGGCGT1158.8665183E-710.7347836
TATACCG803.7860413E-410.6831745
GACGTGG5400.010.5512847
TAGGACG12850.010.4938054
TGTAGGA16050.010.4728872
TAGGACA5650.010.4205594
TGGCGAG14750.010.29703818
AGGACCT11850.010.257455
ATAGGAC2053.6379788E-1210.1914743
TCCTACA12000.010.1297082
GACGTGA6750.010.1292327
Produced by FastQC (version 0.11.5)