##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512796_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2139487 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.74921605038965 31.0 25.0 34.0 10.0 34.0 2 28.270073620452006 31.0 27.0 34.0 16.0 34.0 3 29.42059615225519 31.0 30.0 34.0 20.0 34.0 4 33.249178424547566 35.0 35.0 37.0 27.0 37.0 5 33.59990689356841 37.0 35.0 37.0 28.0 37.0 6 33.86999593827866 37.0 35.0 37.0 30.0 37.0 7 33.85032860681088 37.0 35.0 37.0 30.0 37.0 8 33.891149139957385 37.0 35.0 37.0 30.0 37.0 9 35.4540443573623 39.0 37.0 39.0 30.0 39.0 10 35.38740688772589 39.0 37.0 39.0 30.0 39.0 11 35.42690934789508 39.0 37.0 39.0 30.0 39.0 12 35.33524905736749 39.0 37.0 39.0 30.0 39.0 13 35.31963737101464 39.0 37.0 39.0 30.0 39.0 14 36.5087864520794 40.0 37.0 41.0 30.0 41.0 15 36.41852649723976 40.0 37.0 41.0 29.0 41.0 16 36.32477177940319 40.0 37.0 41.0 29.0 41.0 17 36.27304208906153 40.0 37.0 41.0 28.0 41.0 18 36.18772677749386 40.0 37.0 41.0 27.0 41.0 19 36.21033500086703 40.0 37.0 41.0 27.0 41.0 20 36.130138206027894 40.0 37.0 41.0 27.0 41.0 21 36.03844519737675 40.0 37.0 41.0 27.0 41.0 22 35.91283284263938 40.0 37.0 41.0 27.0 41.0 23 35.77067493282268 40.0 37.0 41.0 27.0 41.0 24 35.64931593414683 40.0 37.0 41.0 26.0 41.0 25 35.53214392048187 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.56357236944876 1101 2 2.1285815849589156 1101 3 0.9797231445771608 1101 4 0.7958771639752555 1101 5 0.46226873451621486 1101 6 0.3124478457467177 1101 7 0.01615259151341064 1101 8 -0.048169037742212595 1101 9 0.007210607635371957 1101 10 -0.13815247371980632 1101 11 -0.010548426678582246 1101 12 -0.05763490167858265 1101 13 -0.028131955456942137 1101 14 -0.24561310342099318 1101 15 -0.36443042549114324 1101 16 -0.481761912630283 1101 17 -0.5352613828864179 1101 18 -0.4971822454388004 1101 19 -0.41772174124795924 1101 20 -0.5072536023794072 1101 21 -0.6653620299558582 1101 22 -0.5066733581515805 1101 23 -0.5825924922755803 1101 24 -0.5742313683538569 1101 25 -0.5649471402283908 1102 1 3.647646382006009 1102 2 2.2251891200182783 1102 3 1.2112528016105664 1102 4 0.9695041561998679 1102 5 0.6579307802390915 1102 6 0.40746370117769715 1102 7 0.1723867473327303 1102 8 0.27201684731048204 1102 9 0.17671985156845693 1102 10 0.20472330081260992 1102 11 0.23595627376736417 1102 12 0.17521860952140145 1102 13 -0.01213633884881915 1102 14 -0.0713664579894484 1102 15 -0.12500509929364512 1102 16 -0.17941614886816382 1102 17 -0.1282206824164689 1102 18 0.023484089037012268 1102 19 -0.010447579886665892 1102 20 -0.04730591834690756 1102 21 -0.10484015758860465 1102 22 -0.1488457226549471 1102 23 -0.39357480897673724 1102 24 -0.3771077374478935 1102 25 -0.29617747094373925 1103 1 2.6472441625408543 1103 2 1.3959070893240941 1103 3 0.3836582781461253 1103 4 -0.07429240240433899 1103 5 -0.2892000416419478 1103 6 -0.4323614694442526 1103 7 -0.4852330055744716 1103 8 -0.5522858273733604 1103 9 -0.9692608645787644 1103 10 -0.8368693673011407 1103 11 -0.8127500840975799 1103 12 -0.738094023118407 1103 13 -0.7272326484852272 1103 14 -1.3065053182113786 1103 15 -1.1390109556695407 1103 16 -1.1755648218841515 1103 17 -1.3363586709108688 1103 18 -1.2471536631912556 1103 19 -1.142132115833519 1103 20 -1.2929667490510681 1103 21 -1.2930546736286175 1103 22 -1.490558376161779 1103 23 -1.5406994312344366 1103 24 -1.4558270767192525 1103 25 -1.3960186244970743 1104 1 -6.584728513911617 1104 2 -8.092044429102739 1104 3 -7.749297046517125 1104 4 -5.86255306858288 1104 5 -3.742233699231761 1104 6 -2.4371127275361353 1104 7 -2.0003540787588108 1104 8 -1.6194237700299396 1104 9 -1.4536015430767222 1104 10 -1.478910991837708 1104 11 -1.4251757026836316 1104 12 -1.4737269334074483 1104 13 -1.5746773854190863 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12 -0.5739590087750415 1107 13 -0.6988978072632364 1107 14 -0.8987910258913487 1107 15 -1.0049008373682469 1107 16 -0.9329410905368078 1107 17 -0.8887769203524982 1107 18 -0.9140197725869896 1107 19 -0.9861010820367397 1107 20 -0.8560649660838848 1107 21 -0.958145361634891 1107 22 -1.1203682805639232 1107 23 -1.1625308642723695 1107 24 -1.2816358296889803 1107 25 -1.1786687330980854 1108 1 2.380058214671223 1108 2 1.165666897087057 1108 3 0.13199286685817668 1108 4 0.009785953505797806 1108 5 -0.3842782366608333 1108 6 -0.6258186650322557 1108 7 -0.9709162184261331 1108 8 -0.8651587555648987 1108 9 -1.1275509133610981 1108 10 -1.1445985779761045 1108 11 -1.2679069157191378 1108 12 -1.280519553545929 1108 13 -1.1545742366340264 1108 14 -1.4941458812720825 1108 15 -1.5457552610395453 1108 16 -1.764980471774102 1108 17 -1.5722127139907727 1108 18 -1.6140529684772176 1108 19 -1.6449293023977347 1108 20 -1.6549939575503956 1108 21 -1.337743487790675 1108 22 -1.8138996419944249 1108 23 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0.43926689624307613 2313 10 0.45840050989083636 2313 11 0.3385626582961123 2313 12 0.4794641585318331 2313 13 0.5114682480889812 2313 14 0.6661316539950661 2313 15 0.6937640992379386 2313 16 0.8004066101325265 2313 17 0.7867553345037095 2313 18 0.8124096297101531 2313 19 0.8043783078254094 2313 20 0.7130450223018983 2313 21 0.7898493970532883 2313 22 0.7423018148278615 2313 23 0.7729553867028613 2313 24 0.9295397899135978 2313 25 0.948538254540253 2314 1 3.798726399618552 2314 2 2.292478872807969 2314 3 1.1491565993522208 2314 4 0.7331510017607741 2314 5 0.4512545576193716 2314 6 -0.7469564019086405 2314 7 -0.28087829492186955 2314 8 -0.1371076384697929 2314 9 -0.2184667868901542 2314 10 -0.045908306701605284 2314 11 -0.2923762274183872 2314 12 -1.1669477330325293 2314 13 -0.6852937566823627 2314 14 -0.6608100786161089 2314 15 -0.4290988156047888 2314 16 -0.38226474248876485 2314 17 -0.21826979661660317 2314 18 -0.2662999374277746 2314 19 -0.28333951015419245 2314 20 -0.011595541691420408 2314 21 -0.3383387709258514 2314 22 -0.576327434150123 2314 23 -0.4527057670160062 2314 24 -0.16336775632989742 2314 25 -0.17142180496828274 2315 1 -0.19751571044507443 2315 2 1.126350050627714 2315 3 0.9645495540809534 2315 4 0.7548190779872357 2315 5 0.6442067937288911 2315 6 0.43726376044877213 2315 7 0.4290170957298187 2315 8 0.26751203140182156 2315 9 -0.5280903764965501 2315 10 -0.20781301343768632 2315 11 0.036098074182476125 2315 12 0.2503867630559853 2315 13 0.19142013989210227 2315 14 0.27332591388776706 2315 15 0.26965771743279987 2315 16 0.2699770193100264 2315 17 0.2528424777177918 2315 18 0.24423507389609966 2315 19 -0.029116360161935972 2315 20 0.1388524104446347 2315 21 0.08297250937924616 2315 22 -0.5646424818241442 2315 23 -0.20860511707377327 2315 24 0.09715891700014367 2315 25 0.3167047162767531 2316 1 0.7676877461759091 2316 2 0.8413615626168962 2316 3 0.42920466444999406 2316 4 0.19439654895055725 2316 5 0.05323711172048462 2316 6 -0.11392174820625911 2316 7 -0.14909118237621044 2316 8 -0.19313515783718316 2316 9 -0.05180066681529638 2316 10 -0.26773384902453756 2316 11 -0.3126946849275427 2316 12 -0.2682989735882444 2316 13 -0.1774999040037244 2316 14 -0.12633117629491153 2316 15 -0.274422733566162 2316 16 -0.12707665696594717 2316 17 -0.05966776421333009 2316 18 0.07605336334179924 2316 19 0.04331949985434136 2316 20 -0.0965824792402401 2316 21 0.01837994658609432 2316 22 0.015579355766433878 2316 23 0.05789538122035509 2316 24 -0.16704774037815895 2316 25 -0.12237153010912039 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82932.0 3 3.0 4 720.0 5 3007.0 6 3716.0 7 3385.0 8 3013.0 9 3034.0 10 2790.0 11 2972.0 12 2896.0 13 2972.0 14 2955.0 15 3027.0 16 3027.0 17 3290.0 18 3780.0 19 4592.0 20 5790.0 21 6680.0 22 7759.0 23 9186.0 24 10643.0 25 12173.0 26 13902.0 27 15594.0 28 18461.0 29 22203.0 30 27936.0 31 36149.0 32 48475.0 33 66702.0 34 96716.0 35 144408.0 36 258501.0 37 359911.0 38 846187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0299181844384 20.467350349625736 11.71795557170538 23.784775894230485 2 14.877420909494518 19.999756215451157 41.095183333012976 24.02763954204135 3 18.774764420140503 24.535508188283888 29.53814957018365 27.15157782139196 4 12.225521346542747 16.69593376416751 38.494983653865084 32.58356123542466 5 13.365704925963811 37.639008300416386 35.6477709448861 13.34751582873371 6 31.92558326026186 36.820435281156556 18.241169593272925 13.012811865308663 7 28.14657603664777 31.53068954553332 22.226960473745834 18.09577394407308 8 25.32600225574021 36.42870497364008 20.630604499953375 17.614688270666335 9 26.559798299581523 15.370662464262308 20.126662391182446 37.942876844973725 10 14.870769295618311 28.91540815920305 34.540579401302494 21.673243143876146 11 34.35112751235091 22.689124453673788 24.239688209849238 18.720059824126064 12 23.14861280378522 24.78327914433391 31.614091857499567 20.454016194381307 13 28.788412729951524 21.746375697774244 25.869991864767695 23.59521970750653 14 21.1489390024861 21.849396099488906 27.810734092536833 29.190930805488165 15 23.33243328911377 30.029137953299447 23.565255162536246 23.073173595050537 16 22.51233099381723 28.109636202190224 27.288760780365173 22.089272023627373 17 22.11822016411581 28.226171104223603 27.520420470387613 22.135188261272972 18 21.894886699119574 27.64193772715941 28.983900384448845 21.47927518927217 19 23.422577681839282 27.06332174857275 27.658159152031125 21.85594141755684 20 23.524413550176124 27.08030427939744 28.109917185040846 21.285364985385595 21 23.341136110634377 26.57281763727777 27.368428950179453 22.717617301908398 22 23.27917894587735 27.416110105530468 27.426006957815485 21.878703990776696 23 22.715881969294514 27.209577610546376 27.599019024593407 22.4755213955657 24 22.87372188786526 26.885583312577715 28.14836912446006 22.092325675096966 25 22.959158062244583 27.0288084918128 27.667871262301443 22.344162183641174 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1866.0 1 1866.0 2 1622.0 3 1378.0 4 1378.0 5 1378.0 6 2212.5 7 3047.0 8 3047.0 9 3047.0 10 4105.5 11 5164.0 12 5164.0 13 5164.0 14 7930.5 15 10697.0 16 10697.0 17 10697.0 18 18521.0 19 26345.0 20 26345.0 21 26345.0 22 40467.5 23 54590.0 24 54590.0 25 54590.0 26 76603.0 27 98616.0 28 98616.0 29 98616.0 30 125565.5 31 152515.0 32 152515.0 33 152515.0 34 174615.0 35 196715.0 36 196715.0 37 196715.0 38 215020.0 39 233325.0 40 233325.0 41 233325.0 42 247298.0 43 261271.0 44 261271.0 45 261271.0 46 270749.5 47 280228.0 48 280228.0 49 280228.0 50 272113.0 51 263998.0 52 263998.0 53 263998.0 54 237025.0 55 210052.0 56 210052.0 57 210052.0 58 180148.0 59 150244.0 60 150244.0 61 150244.0 62 122991.0 63 95738.0 64 95738.0 65 95738.0 66 74504.0 67 53270.0 68 53270.0 69 53270.0 70 38850.0 71 24430.0 72 24430.0 73 24430.0 74 17207.0 75 9984.0 76 9984.0 77 9984.0 78 6919.5 79 3855.0 80 3855.0 81 3855.0 82 2592.5 83 1330.0 84 1330.0 85 1330.0 86 898.0 87 466.0 88 466.0 89 466.0 90 333.5 91 201.0 92 201.0 93 201.0 94 149.0 95 97.0 96 97.0 97 97.0 98 81.0 99 65.0 100 65.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7271897422139044 2 0.30175457948564305 3 0.2867042426525611 4 0.29264024506809344 5 0.2963794591881138 6 0.21458415031266842 7 0.4788063680686071 8 0.2512751888653682 9 0.22603549355523075 10 0.20341324812910758 11 0.2444978632728313 12 0.24725553368634628 13 0.2598286411649148 14 0.2815160830610329 15 0.4656256382955353 16 0.5666778998890856 17 0.5592462118255451 18 0.2581459948109056 19 0.22369848473021803 20 0.2153319931366725 21 0.32311484014625935 22 0.35036436304590773 23 0.45665152440748646 24 0.3521404897529174 25 0.37055611929401766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2139487.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.20147014606479 #Duplication Level Percentage of deduplicated Percentage of total 1 87.9023115060641 68.74089989011559 2 8.484109549295429 13.269396792703395 3 1.8689012484431384 4.38452475558208 4 0.6666248618548587 2.085241769318692 5 0.3156291958377842 1.234133356776746 6 0.18460688058813146 0.8661917676642545 7 0.12770990257639409 0.6990971493579309 8 0.08825793600402508 0.5521520278057652 9 0.06021795754895654 0.42382195285695495 >10 0.2769487784942911 3.6496356797523193 >50 0.012520756975708893 0.6806467435494225 >100 0.01005660381855136 1.5111504426110967 >500 0.001262893499226859 0.6611285158686783 >1k 8.419289994841412E-4 1.2419791560370481 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4664 0.217996183197187 No Hit GGTATCAACGCAGAGTACTTTTTTT 2856 0.13348994408472684 No Hit TATCAACGCAGAGTACTTTTTTTTT 2590 0.12105705713565916 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.67401765002545E-5 0.0 3 0.0 0.0 0.0 4.67401765002545E-5 0.0 4 0.0 0.0 0.0 4.67401765002545E-5 0.0 5 0.0 0.0 0.0 4.67401765002545E-5 0.0 6 0.0 0.0 0.0 4.67401765002545E-5 0.0 7 0.0 0.0 0.0 4.67401765002545E-5 0.0 8 0.0 0.0 0.0 4.67401765002545E-5 0.0 9 0.0 0.0 0.0 4.67401765002545E-5 0.0 10 0.0 0.0 0.0 4.67401765002545E-5 0.0 11 0.0 0.0 0.0 4.67401765002545E-5 0.0 12 0.0 0.0 0.0 4.67401765002545E-5 0.0 13 0.0 0.0 0.0 4.67401765002545E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 605 0.0 13.96955 4 GTAGGAC 1390 0.0 11.272944 3 TTAGGAC 870 0.0 11.243103 3 ATACCGT 85 5.3467644E-5 11.1719475 6 ACCGTCC 60 0.006058522 11.038089 8 AGGACGT 1195 0.0 10.966271 5 GTGTAGG 450 0.0 10.900231 1 GGACGTG 1165 0.0 10.841103 6 GGCGAGG 580 0.0 10.80419 19 AAGGCGT 115 8.8665183E-7 10.734783 6 TATACCG 80 3.7860413E-4 10.683174 5 GACGTGG 540 0.0 10.551284 7 TAGGACG 1285 0.0 10.493805 4 TGTAGGA 1605 0.0 10.472887 2 TAGGACA 565 0.0 10.420559 4 TGGCGAG 1475 0.0 10.297038 18 AGGACCT 1185 0.0 10.25745 5 ATAGGAC 205 3.6379788E-12 10.191474 3 TCCTACA 1200 0.0 10.129708 2 GACGTGA 675 0.0 10.129232 7 >>END_MODULE