FastQCFastQC Report
Thu 26 May 2016
SRR1512796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139487
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57580.2691299362884654No Hit
GGTATCAACGCAGAGTACTTTTTTT35810.16737657204741135No Hit
TATCAACGCAGAGTACTTTTTTTTT34590.16167427051438032No Hit
GTCCTACAGTGGACATTTCTAAATT22470.10502517659607186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG5350.011.18007719
TAGGACC4850.010.5852024
GTAGGAC12300.010.4341123
TGTAGGA13850.010.159192
TGGCGAG11800.010.13786518
CTGTAGG14750.010.12793351
GTATTAA2500.09.8957011
GTATTAG2151.0913936E-119.7363791
GTCCTAA6400.09.6637711
TATGGCG12800.09.64253916
GTCCTAC13550.09.620431
ATGGCGA12900.09.5677917
TTAGGAC8650.09.5615863
GACCGTG1106.845189E-59.4990777
ACCGTGG900.00111486479.4990778
AGGACCT9500.09.4076665
TAGGACG11650.09.3846714
GTCTATA2451.8189894E-129.3209111
TAGGACA7100.09.2392754
GTATTAT3200.09.2177511
Produced by FastQC (version 0.11.5)