FastQCFastQC Report
Thu 26 May 2016
SRR1512795_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512795_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927796
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41560.2155829766220077No Hit
GTATCAACGCAGAGTACTTTTTTTT41270.21407866807483777No Hit
GTCCTAAAGTGTGTATTTCTCATTT36900.19141029445024266No Hit
CTGTAGGACGTGGAATATGGCAAGA36800.19089156736501164No Hit
CTTTAGGACGTGAAATATGGCGAGG32180.16692637602733898No Hit
GGTATCAACGCAGAGTACTTTTTTT24510.12714000859012053No Hit
GTCCTACAGTGTGCATTTCTCATTT22970.11915161147756298No Hit
TATCAACGCAGAGTACTTTTTTTTT22350.11593550354913071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1650.013.23234718
TAGGACC7650.013.0326934
CCAACGA1102.7739588E-912.94637719
GCGTGCG751.5160205E-512.6362499
CGTGCGC701.1005449E-412.2030810
AGTGCGG550.003090061712.07981610
GCGTGTA550.003124649412.0618749
CAAGACG600.005898424411.0777894
AGGACCT15750.010.9722875
AAGGCGT1303.2910975E-810.9560566
GGACCTG15600.010.8951896
GTCCATA1157.4779746E-710.8774521
GGCGAGG9350.010.86480219
GTGTAGG4000.010.8251561
GTATTAC2050.010.7958191
TGTAGGA25750.010.7688332
AATCCCG2750.010.70233719
GTAGGAC24150.010.5778693
TGGCGAA909.5905365E-510.54752318
ACTGTTC6150.010.46112258
Produced by FastQC (version 0.11.5)