FastQCFastQC Report
Thu 26 May 2016
SRR1512795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927796
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51900.26921935723489415No Hit
GTCCTACAGTGGACATTTCTAAATT45250.23472400606703198No Hit
CTGTAGGACGTGGAATATGGCAAGA41980.21776163037997795No Hit
GTCCTAAAGTGTGTATTTCTCATTT38890.20173296344633976No Hit
CTTTAGGACGTGAAATATGGCGAGG36690.19032096757125755No Hit
GGTATCAACGCAGAGTACTTTTTTT31280.16225783226025992No Hit
TATCAACGCAGAGTACTTTTTTTTT30390.1576411612017039No Hit
GTCCTACAGTGTGCATTTCTCATTT26010.13492091486858568No Hit
CTGAAGGACCTGGAATATGGCGAGA21220.11007388748602032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1350.014.77039318
TCGAACT853.966803E-612.28797419
TAGGACC7900.012.0349184
CCAACGA1353.7471182E-1011.95698519
CTCGAAC802.881901E-511.86906518
GGCGAGG9700.011.35514719
AATCCCG3100.011.33304419
AGACCGT600.00588477511.0812446
GGTATCA12350.010.9423141
GTATAGG2750.010.7279511
GGACATG6850.010.676826
TGTAGGA25250.010.5815422
TTAGGAC13750.010.4410563
TAGGACA7800.010.3608294
TAGAAAT6950.010.2599834
ACTGTTC6300.010.2515048
TGGCGAG24150.010.22263218
AGGACGT23550.010.1739855
CCGTCCA750.002653350610.13089759
GTAGGAC25250.010.0912183
Produced by FastQC (version 0.11.5)