FastQCFastQC Report
Thu 26 May 2016
SRR1512794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809766
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48520.2681009589084998No Hit
GGTATCAACGCAGAGTACTTTTTTT29300.16189938367722678No Hit
TATCAACGCAGAGTACTTTTTTTTT27410.15145604459361045No Hit
GTCCTACAGTGGACATTTCTAAATT26070.14405177243908882No Hit
GTCCTAAAGTGTGTATTTCTCATTT25210.13929977687723163No Hit
CTGTAGGACGTGGAATATGGCAAGA23500.1298510415158645No Hit
CTTTAGGACGTGAAATATGGCGAGG22220.12277830393542591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC400.00542751414.1907398
TAGGACC5250.013.2032584
AGCACCG1001.4513171E-712.3444845
ATAGGAC2500.011.3961773
AGGACGT14900.011.3439255
GGCGAGG7250.011.13543519
TCCAACG1451.2241799E-911.13419718
GTAGGAC14100.011.1132943
GGACGTG14650.011.0840946
GCACCGT600.00589461411.07869156
TGTAGGA15350.010.8888562
TTAGGAC10150.010.6663723
TAGGACG16000.010.6230844
GTCCTAC15150.010.6038461
GTATTAT2001.8189894E-1210.5816221
TAGGACA5300.010.5704564
GACGTGA8350.010.4623917
AATGTCC4450.010.41717158
TGGCGAG15850.010.24577418
CGTGGAA7500.010.2357719
Produced by FastQC (version 0.11.5)