FastQCFastQC Report
Thu 26 May 2016
SRR1512794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809766
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58130.32120174652413624No Hit
GGTATCAACGCAGAGTACTTTTTTT37990.20991664115692305No Hit
TATCAACGCAGAGTACTTTTTTTTT36070.19930753478626517No Hit
GTCCTACAGTGGACATTTCTAAATT28210.15587650558138455No Hit
CTGTAGGACGTGGAATATGGCAAGA27900.1541635769486221No Hit
GTCCTAAAGTGTGTATTTCTCATTT26910.14869325647625165No Hit
CTTTAGGACGTGAAATATGGCGAGG25370.14018386907478647No Hit
GTACATGGGAAGCAGTGGTATCAAC20280.11205868604007369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG602.549433E-514.2613935
TAGGACC6000.012.0429544
TCCAACG1701.2732926E-1111.16964418
TGGACCG600.005841435411.0921945
CCGTCCA600.005883678311.0814629
GTACCGT700.001492675910.8562116
AGACGTG803.7435666E-410.6960455
CCAACGA1355.7410944E-810.54881719
GTAGGAC16600.010.5379573
TGTAGGA18600.010.4802512
GGCGAGG5800.010.31237819
CTGTAGG17700.010.2715471
AGGACGT15900.010.225155
GTATAAG2053.6379788E-1210.2151441
GTCCTAC14950.010.1871931
GGACGTG15500.010.173326
GTCCTAT2250.010.1532361
GACGTGG8150.010.1393937
GTCTAGA2002.3646862E-119.9945921
TAGGACG16700.09.67839054
Produced by FastQC (version 0.11.5)