FastQCFastQC Report
Thu 26 May 2016
SRR1512793_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512793_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572727
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47970.30501161358582896No Hit
GGTATCAACGCAGAGTACTTTTTTT28400.1805780659961964No Hit
GTACATGGGAAGCAGTGGTATCAAC27410.17428326721675153No Hit
TATCAACGCAGAGTACTTTTTTTTT25920.16480927713455673No Hit
CCCATGTACTCTGCGTTGATACCAC18540.11788441350596766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT802.8890474E-511.86586512
TACACCG658.053792E-411.687055
GACCGTG907.4979616E-611.605897
TAGGACC1751.8189894E-1211.3948754
TTAGGAC2950.011.26647953
GTCCTAT1201.07358574E-711.2219691
AGGACGT5000.011.204965
CTAATGG600.005893979711.0787043
CGGTACT600.00589538311.078354
GATCTAG803.3613003E-410.8211831
TAGGACG5450.010.802484
GGACGTG5150.010.3254536
GCTCTAT1408.0734026E-810.3058891
GTGTAGG2800.010.3058891
GTAGGAC6000.010.2873693
TCCAACG1201.5358364E-610.28506518
TAGACAG2700.010.1991175
GTGTTAT1701.2551027E-910.1846431
GTATTAT1701.2551027E-910.1846431
GTACTAA855.880588E-410.1846431
Produced by FastQC (version 0.11.5)