FastQCFastQC Report
Thu 26 May 2016
SRR1512793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572727
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55930.3556243391256079No Hit
GGTATCAACGCAGAGTACTTTTTTT36510.2321445489268004No Hit
TATCAACGCAGAGTACTTTTTTTTT34370.21853761015103068No Hit
GTACATGGGAAGCAGTGGTATCAAC30900.1964740225099461No Hit
CCCATGTACTCTGCGTTGATACCAC21310.13549713332320232No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16840.10707516307661787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAA752.032313E-411.4248521
TAGGACC2200.011.2356274
GAAGCGA600.005882157511.08176
TCGAACT951.3695577E-510.99313319
TATACTG2250.010.9862965
GGCTTAC700.001464564410.8808131
TTAGACC700.001482191710.8652224
GTAGGAC6600.010.3713483
TTAAGCT1752.3283064E-1010.321964
GTCTAGC856.462326E-410.0807531
CTTATGC856.548096E-410.0663083
GTGTAGC2850.010.0218011
GTATAAG2002.3646862E-119.9967461
CCTCGAA951.6542409E-49.99375717
CGTGAAA4200.09.9502829
AGGACGT6800.09.9268155
GGACGTG6650.09.855696
TTACACA2900.09.8348994
GTATTAC1659.855285E-99.8092171
GGGTGAT1651.0242729E-89.7861257
Produced by FastQC (version 0.11.5)