FastQCFastQC Report
Thu 26 May 2016
SRR1512792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921303
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50480.2627383603731426No Hit
CTGTAGGACGTGGAATATGGCAAGA45600.23733893092344102No Hit
GTCCTAAAGTGTGTATTTCTCATTT44410.2311452175945179No Hit
GTATCAACGCAGAGTACTTTTTTTT41010.21344889379759463No Hit
CTTTAGGACGTGAAATATGGCGAGG38500.2003848429945719No Hit
GTCCTACAGTGTGCATTTCTCATTT27970.1455782872352773No Hit
GGTATCAACGCAGAGTACTTTTTTT23930.1245508907236391No Hit
GTACATGGGAAGCAGTGGTATCAAC23290.12121981800892415No Hit
TATCAACGCAGAGTACTTTTTTTTT21970.11434948053482455No Hit
CTGAAGGACCTGGAATATGGCGAGA20520.10680251891554847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCA500.001509770313.28827110
GGACCGT604.110629E-412.6601366
GGCGAGG11700.011.92911819
TAGGACC8300.011.7830794
CCCGTTT1052.7447823E-711.75399312
CGTAGGA658.054374E-411.6871982
ATAGGAC2800.011.5306733
TAGGACA6450.011.1880284
AGTGCGG600.00591520911.07355810
CCCTATA951.3671412E-510.99519352
AGGACTG3500.010.8512625
TCCAACG1158.9043715E-710.73106418
TTAGGAC16200.010.6095593
ACCTTTT6300.010.55647415
GTCCTAA11850.010.5514281
ATAAGAC2001.8189894E-1210.4454343
AGGACCT15700.010.4020065
GTACTAG1408.0879545E-810.3050351
TTTAGGA18100.010.2303342
TAAGACC1951.4551915E-1110.2254944
Produced by FastQC (version 0.11.5)