##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512792_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1921303 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.770498458598148 31.0 25.0 34.0 10.0 34.0 2 28.316082367018634 31.0 27.0 34.0 16.0 34.0 3 29.47501357151891 31.0 30.0 34.0 22.0 34.0 4 33.30010570951068 35.0 35.0 37.0 28.0 37.0 5 33.660970705817874 37.0 35.0 37.0 28.0 37.0 6 33.94228135801589 37.0 35.0 37.0 30.0 37.0 7 33.92548078049116 37.0 35.0 37.0 30.0 37.0 8 33.9656306163057 37.0 35.0 37.0 30.0 37.0 9 35.54523778914622 39.0 37.0 39.0 30.0 39.0 10 35.469103519850854 39.0 37.0 39.0 30.0 39.0 11 35.50619449404909 39.0 37.0 39.0 30.0 39.0 12 35.415337924314905 39.0 37.0 39.0 30.0 39.0 13 35.40467432778692 39.0 37.0 39.0 30.0 39.0 14 36.5924422123944 40.0 38.0 41.0 30.0 41.0 15 36.5092445075035 40.0 37.0 41.0 30.0 41.0 16 36.42091643015183 40.0 37.0 41.0 29.0 41.0 17 36.377714498962426 40.0 37.0 41.0 29.0 41.0 18 36.29903300000052 40.0 37.0 41.0 29.0 41.0 19 36.31605686349316 40.0 37.0 41.0 28.0 41.0 20 36.24396984754617 40.0 37.0 41.0 28.0 41.0 21 36.14614196719622 40.0 37.0 41.0 27.0 41.0 22 36.03540618007675 40.0 37.0 41.0 27.0 41.0 23 35.88891601168582 40.0 37.0 41.0 27.0 41.0 24 35.781480068474366 40.0 37.0 41.0 27.0 41.0 25 35.660663622551986 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.219824560075079 1101 2 1.8474954415445204 1101 3 0.6886266672651011 1101 4 0.4195291517467368 1101 5 0.09510111498755691 1101 6 -0.15427905684221344 1101 7 -0.19953500749976172 1101 8 -0.34258482328479545 1101 9 -0.38858580931202624 1101 10 -0.4269971224517306 1101 11 -0.3190554837965891 1101 12 -0.46557950836666606 1101 13 -0.5267234565830563 1101 14 -0.5844747623699362 1101 15 -0.5443483714336566 1101 16 -0.7267221550097247 1101 17 -0.7525262413214975 1101 18 -0.6896816775537644 1101 19 -0.7126271601857468 1101 20 -0.8760018342783198 1101 21 -0.8859508457407443 1101 22 -1.069652564650987 1101 23 -0.7825850806085839 1101 24 -0.9786483961293513 1101 25 -1.1123094489448206 1102 1 3.6009564318306175 1102 2 2.091140108896827 1102 3 1.0326593029568798 1102 4 0.8054550096663533 1102 5 0.4573895470803748 1102 6 0.3208922416843478 1102 7 0.12018625222308543 1102 8 0.12680494580819612 1102 9 0.1857376530586876 1102 10 0.08850080807432192 1102 11 -0.11070448506093555 1102 12 0.004158239065013447 1102 13 0.08365083675786877 1102 14 -0.07712303226083606 1102 15 -0.22133610313410657 1102 16 -0.44672716149649006 1102 17 -0.24687657917339578 1102 18 -0.25930880776058984 1102 19 -0.2580044639519272 1102 20 -0.32310895487103863 1102 21 -0.30184926082319663 1102 22 -0.30242084580648054 1102 23 -0.5087790812832225 1102 24 -0.5438951942644294 1102 25 -0.4450781771080585 1103 1 2.4573687870649366 1103 2 1.2089613982417262 1103 3 0.21431196645578154 1103 4 -0.12463839570283852 1103 5 -0.3431297052342188 1103 6 -0.5564103010085901 1103 7 -0.7520615603909846 1103 8 -0.7111197515448211 1103 9 -1.2182245296972667 1103 10 -1.0389644157683051 1103 11 -0.9248494490561754 1103 12 -0.9713746424376879 1103 13 -1.1091365178838757 1103 14 -1.6821904039054871 1103 15 -1.5622943022166424 1103 16 -1.6797406105422894 1103 17 -1.601828043461758 1103 18 -1.635843217312555 1103 19 -1.8406213628810306 1103 20 -1.7022863997893651 1103 21 -1.8287822747463025 1103 22 -1.953770253944171 1103 23 -1.9044607224112937 1103 24 -1.987419157142476 1103 25 -2.123237501922034 1104 1 -7.051872940736349 1104 2 -8.742178597496608 1104 3 -8.338820358283193 1104 4 -6.603144377255909 1104 5 -4.3348103237846445 1104 6 -3.0942660740444197 1104 7 -2.7294047158504817 1104 8 -2.1379712933548127 1104 9 -2.0682793225498415 1104 10 -2.219529695364052 1104 11 -1.9445550896759443 1104 12 -1.9729240625466602 1104 13 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0.24481184183223093 2314 20 0.15927347562549699 2314 21 0.13006194352465883 2314 22 -0.21575943888113613 2314 23 -0.029302589846729177 2314 24 0.11454551324507634 2314 25 0.20611181058816186 2315 1 -0.2219855441822851 2315 2 1.093558581990571 2315 3 0.9670497746702758 2315 4 0.6822222622773992 2315 5 0.578016972375984 2315 6 0.4131540521257051 2315 7 0.32567261422684624 2315 8 0.2281153977318766 2315 9 -0.5766474205012742 2315 10 -0.4001751301761587 2315 11 -0.06703507882259885 2315 12 -0.10304378849972551 2315 13 -0.03270723194974323 2315 14 -0.007500542067653271 2315 15 0.04699402581591272 2315 16 0.23964954369319713 2315 17 0.2131652450714654 2315 18 0.12911394899502682 2315 19 -0.04542818378318714 2315 20 0.05702201989532796 2315 21 0.037154466473218406 2315 22 -0.6703672410513164 2315 23 -0.3253470635076994 2315 24 0.008058511199919849 2315 25 0.1269104068362168 2316 1 1.2274408812797866 2316 2 1.172255595478049 2316 3 0.7059328653717643 2316 4 0.5180086748942543 2316 5 0.2641209472564512 2316 6 0.1394639964631068 2316 7 0.026954459656089114 2316 8 -0.07804227728417601 2316 9 -0.0388900701837116 2316 10 0.01530959829026557 2316 11 -0.1254585070677976 2316 12 -0.08229965804942907 2316 13 0.015121123862719799 2316 14 0.09774770955583989 2316 15 0.03471524896584555 2316 16 0.18764426288252167 2316 17 0.23573580455194332 2316 18 0.23682643026242545 2316 19 0.06793307005253979 2316 20 0.025479880453374903 2316 21 0.15185376355449876 2316 22 0.11839141162833755 2316 23 0.2305742238683095 2316 24 0.20329686130340008 2316 25 0.2421248505670377 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71780.0 3 7.0 4 602.0 5 2604.0 6 3119.0 7 2936.0 8 2656.0 9 2657.0 10 2564.0 11 2540.0 12 2646.0 13 2577.0 14 2601.0 15 2597.0 16 2697.0 17 2840.0 18 3163.0 19 3949.0 20 4931.0 21 5656.0 22 6838.0 23 8257.0 24 9381.0 25 10710.0 26 12264.0 27 13970.0 28 16248.0 29 19642.0 30 24987.0 31 32115.0 32 42945.0 33 59986.0 34 86792.0 35 130365.0 36 231369.0 37 322299.0 38 768013.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41227327760894 20.836109489847477 11.720076563660225 24.031540668883355 2 14.315706483715005 19.998955755354363 42.37565656882094 23.30968119210969 3 18.804326376424818 24.682407677135963 29.56513840207235 26.94812754436687 4 12.068954715511419 16.721659474094732 38.71850400072674 32.49088180966711 5 12.925655941666692 38.043468045848826 36.052122393018884 12.9787536194656 6 31.759567322589426 37.02651129841825 18.32170575390429 12.892215625088028 7 28.09694601028231 31.347030876747738 22.431213702913496 18.124809410056454 8 24.65147516739368 37.70398128467962 20.68741125434498 16.95713229358172 9 26.628564571834012 15.1373136197164 20.43870449399514 37.79541731445445 10 15.160061528222082 28.769346885955283 34.880493060340534 21.190098525482103 11 34.05424529409524 22.059582470808074 25.333430044005095 18.55274219109159 12 23.993358196610533 24.39474688039275 31.97604609186123 19.635848831135487 13 29.18822298360476 22.127388634756446 25.721346168398913 22.963042213239877 14 20.536995082634704 22.763225206978273 27.633765908355347 29.066013802031677 15 22.627986508770398 31.97791697632445 23.15318005687069 22.240916458034462 16 21.522879950412115 27.980717684111774 29.004462490786036 21.491939874690075 17 21.56043123061122 28.975151995493793 28.17745702927464 21.286959744620347 18 21.344460188352812 27.23569152725479 30.885059900787283 20.53478838360511 19 23.688830388030766 26.710928374185677 27.70170405487116 21.898537182912392 20 23.725298102514504 27.394288242100245 28.455918178697708 20.424495476687543 21 22.533298028518562 26.818707164840582 27.386508860411762 23.261485946229097 22 23.088672874074323 28.622421474064293 27.292411945637816 20.996493706223568 23 21.673591156176215 28.333076225164007 28.245014498286093 21.748318120373686 24 23.138829424344905 26.864095917789527 29.015632819413405 20.98144183845216 25 22.426654419544402 27.595890944183115 28.36974814503644 21.607706491236044 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1777.0 1 1777.0 2 1554.5 3 1332.0 4 1332.0 5 1332.0 6 2016.5 7 2701.0 8 2701.0 9 2701.0 10 3552.0 11 4403.0 12 4403.0 13 4403.0 14 6662.5 15 8922.0 16 8922.0 17 8922.0 18 15850.5 19 22779.0 20 22779.0 21 22779.0 22 36351.5 23 49924.0 24 49924.0 25 49924.0 26 72868.5 27 95813.0 28 95813.0 29 95813.0 30 124842.5 31 153872.0 32 153872.0 33 153872.0 34 173023.0 35 192174.0 36 192174.0 37 192174.0 38 205517.0 39 218860.0 40 218860.0 41 218860.0 42 229354.0 43 239848.0 44 239848.0 45 239848.0 46 247267.5 47 254687.0 48 254687.0 49 254687.0 50 242084.0 51 229481.0 52 229481.0 53 229481.0 54 201147.0 55 172813.0 56 172813.0 57 172813.0 58 147431.0 59 122049.0 60 122049.0 61 122049.0 62 99157.0 63 76265.0 64 76265.0 65 76265.0 66 58751.0 67 41237.0 68 41237.0 69 41237.0 70 30334.5 71 19432.0 72 19432.0 73 19432.0 74 13712.0 75 7992.0 76 7992.0 77 7992.0 78 5535.0 79 3078.0 80 3078.0 81 3078.0 82 2100.5 83 1123.0 84 1123.0 85 1123.0 86 773.0 87 423.0 88 423.0 89 423.0 90 301.0 91 179.0 92 179.0 93 179.0 94 125.5 95 72.0 96 72.0 97 72.0 98 69.5 99 67.0 100 67.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7280460187695537 2 0.31452613148472675 3 0.3017223207375411 4 0.307135313898953 5 0.3104663866136679 6 0.2242748801204183 7 0.49320695382248403 8 0.2608646319711154 9 0.23541315451024641 10 0.21516647816611956 11 0.25680488710005656 12 0.25794994334573984 13 0.2705455620482558 14 0.29370692701775825 15 0.48123591125397713 16 0.580803756617254 17 0.5750784753888377 18 0.27158652227160424 19 0.235361106499079 20 0.22614860852244542 21 0.3409144731466094 22 0.365949566518139 23 0.4688484845961309 24 0.3641799341384466 25 0.3843225144602387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1921303.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.63419497297801 #Duplication Level Percentage of deduplicated Percentage of total 1 86.2138787353052 63.482895561721456 2 9.662292063944696 14.229501954447235 3 2.2118252390543556 4.885979126960463 4 0.7707346061802252 2.2700968905558447 5 0.36131442071326214 1.330254825067447 6 0.17923509410750735 0.7918699119307353 7 0.11571110554163358 0.5964205874194037 8 0.08656782249191686 0.5099481535804764 9 0.060516578904697006 0.401048061314946 >10 0.29762537394432115 3.7912814408389797 >50 0.019118429613129876 0.9960849617380956 >100 0.017116624689496155 2.6784683888018077 >500 0.002210194224538842 1.1757076818365784 >1k 0.0017824146972087408 2.595429175022849 >5k 7.129658788834963E-5 0.26501327876360975 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5048 0.2627383603731426 No Hit CTGTAGGACGTGGAATATGGCAAGA 4560 0.23733893092344102 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4441 0.2311452175945179 No Hit GTATCAACGCAGAGTACTTTTTTTT 4101 0.21344889379759463 No Hit CTTTAGGACGTGAAATATGGCGAGG 3850 0.2003848429945719 No Hit GTCCTACAGTGTGCATTTCTCATTT 2797 0.1455782872352773 No Hit GGTATCAACGCAGAGTACTTTTTTT 2393 0.1245508907236391 No Hit GTACATGGGAAGCAGTGGTATCAAC 2329 0.12121981800892415 No Hit TATCAACGCAGAGTACTTTTTTTTT 2197 0.11434948053482455 No Hit CTGAAGGACCTGGAATATGGCGAGA 2052 0.10680251891554847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.204801116742128E-5 0.0 10 0.0 5.204801116742128E-5 0.0 5.204801116742128E-5 0.0 11 0.0 5.204801116742128E-5 0.0 5.204801116742128E-5 0.0 12 0.0 5.204801116742128E-5 0.0 5.204801116742128E-5 0.0 13 0.0 5.204801116742128E-5 0.0 5.204801116742128E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCCA 50 0.0015097703 13.288271 10 GGACCGT 60 4.110629E-4 12.660136 6 GGCGAGG 1170 0.0 11.929118 19 TAGGACC 830 0.0 11.783079 4 CCCGTTT 105 2.7447823E-7 11.753993 12 CGTAGGA 65 8.054374E-4 11.687198 2 ATAGGAC 280 0.0 11.530673 3 TAGGACA 645 0.0 11.188028 4 AGTGCGG 60 0.005915209 11.073558 10 CCCTATA 95 1.3671412E-5 10.9951935 2 AGGACTG 350 0.0 10.851262 5 TCCAACG 115 8.9043715E-7 10.731064 18 TTAGGAC 1620 0.0 10.609559 3 ACCTTTT 630 0.0 10.556474 15 GTCCTAA 1185 0.0 10.551428 1 ATAAGAC 200 1.8189894E-12 10.445434 3 AGGACCT 1570 0.0 10.402006 5 GTACTAG 140 8.0879545E-8 10.305035 1 TTTAGGA 1810 0.0 10.230334 2 TAAGACC 195 1.4551915E-11 10.225494 4 >>END_MODULE