FastQCFastQC Report
Thu 26 May 2016
SRR1512792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921303
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55060.28657634948782157No Hit
CTGTAGGACGTGGAATATGGCAAGA51480.26794316148988473No Hit
GTATCAACGCAGAGTACTTTTTTTT50240.2614892081051245No Hit
GTCCTAAAGTGTGTATTTCTCATTT47370.2465514289000746No Hit
CTTTAGGACGTGAAATATGGCGAGG45610.23739097893460845No Hit
GTCCTACAGTGTGCATTTCTCATTT30530.15890257809413716No Hit
GGTATCAACGCAGAGTACTTTTTTT30520.15885053008296973No Hit
TATCAACGCAGAGTACTTTTTTTTT28740.14958598409516874No Hit
GTACATGGGAAGCAGTGGTATCAAC26160.13615759721397405No Hit
CTGAAGGACCTGGAATATGGCGAGA24540.1277258194048518No Hit
CTGTAGGACCTGGAATATGGCGAGA21030.10945696748508695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG400.005251389514.2615545
CGGCTTG400.005284170614.2481829
TAGGACC7700.012.9647114
GGCGAGG11150.010.9839119
CTACACT3050.010.5984744
GTCCTAC26200.010.4593941
AATGTCC6200.010.4180278
GTCCTAA14850.010.2520061
TATACTG3900.010.2390645
TCTAAAC1606.0936145E-910.101673
TCTACAC3500.010.0507373
TGTAGGA26150.09.9995822
TCCAACG1054.134921E-59.94593218
AGGACCT16950.09.8723055
CACCTTT6550.09.8572314
TCCTACA28650.09.8571812
TAGAAAT6950.09.849454
GGACCTG16800.09.7249516
CTATACT2350.09.7097424
TTTAGAA7150.09.7081772