FastQCFastQC Report
Thu 26 May 2016
SRR1512791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533603
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40380.26330151936322505No Hit
GTATCAACGCAGAGTACTTTTTTTT40290.26271466605112276No Hit
GTCCTAAAGTGTGTATTTCTCATTT37980.2476520977071641No Hit
CTGTAGGACGTGGAATATGGCAAGA37390.2438049482167158No Hit
CTTTAGGACGTGAAATATGGCGAGG31630.2062463362421696No Hit
GGTATCAACGCAGAGTACTTTTTTT25270.16477536885360813No Hit
TATCAACGCAGAGTACTTTTTTTTT22690.14795224057334266No Hit
GTCCTACAGTGTGCATTTCTCATTT20790.13556311509562777No Hit
GTACATGGGAAGCAGTGGTATCAAC20610.13438940847142317No Hit
CTGAAGGACCTGGAATATGGCGAGA16930.11039362859879642No Hit
CCCATGTACTCTGCGTTGATACCAC15510.10113438745229372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGA250.006172335718.9245038
TACGGCG456.746194E-414.78240316
TAGGACC7950.012.4201384
ATGTAGG1900.012.1510531
GGCGAGG10250.011.95080919
GAAGCGA658.064187E-411.6852196
TCCAACG907.518949E-611.60292818
AATGTCC5100.011.503138
GTCTAGA1603.6379788E-1111.4232561
GTCTTAT1603.6379788E-1111.4232561
GTCCATA854.6853813E-511.3171581
GGACCTG14050.011.2173946
GAAATGT5650.011.0906166
TAGATTG951.3692066E-510.9933315
AGGACCT15300.010.9835335
GTCCTAC20500.010.8396281
ACTGTTC4500.010.7238858
AGGACGT22950.010.4664165
GACGTGA12500.010.4060297
TGTAGGA23400.010.3882252
Produced by FastQC (version 0.11.5)