FastQCFastQC Report
Thu 26 May 2016
SRR1512791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533603
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45000.29342665605114227No Hit
CTGTAGGACGTGGAATATGGCAAGA42850.2794073824842544No Hit
GTCCTACAGTGGACATTTCTAAATT41840.27282158420399544No Hit
GTCCTAAAGTGTGTATTTCTCATTT38510.2511080116562109No Hit
CTTTAGGACGTGAAATATGGCGAGG36120.2355237959237169No Hit
GGTATCAACGCAGAGTACTTTTTTT28720.1872714124841957No Hit
TATCAACGCAGAGTACTTTTTTTTT28560.186228117707125No Hit
GTACATGGGAAGCAGTGGTATCAAC23800.1551900980892708No Hit
GTCCTACAGTGTGCATTTCTCATTT23580.15375556777079857No Hit
CTGAAGGACCTGGAATATGGCGAGA19960.13015102343957333No Hit
CTGTAGGACCTGGAATATGGCGAGA18390.11991369343956682No Hit
CCCATGTACTCTGCGTTGATACCAC16790.10948074566885956No Hit
ATTTAGAAATGTCCACTGTAGGACG16130.10517715471344277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT400.00529194314.24476513
TAGGACC7500.012.929174
TGTCGAG905.4439806E-712.65912318
GTCTTAC802.7993075E-511.9024711
ATCCCGT1001.9340787E-611.39767110
CGTTTCC1001.9424442E-611.39358314
CCAACGA1001.9432064E-611.39321119
GGCGAGG9750.011.29583319
TCCAACG1354.771209E-911.25255418
CGTGCGC600.0058845411.08106910
AGGACCT14700.010.994915
TCTAGAC1803.45608E-1110.5630473
TGGCGAG23600.010.25871818
TGTAGGA22650.010.2432662
TAGGCTG1303.799105E-710.2386985
GTCCTAA12250.010.1049551
GGACCTG15650.010.0746186
GTATTAC1054.0012048E-59.9754041
TCCCGTT1151.0286036E-59.91101811
CCCGTTT1151.0294016E-59.91037212
Produced by FastQC (version 0.11.5)