FastQCFastQC Report
Thu 26 May 2016
SRR1512790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661260
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42710.25709401297810097No Hit
GGTATCAACGCAGAGTACTTTTTTT25940.15614653937372838No Hit
TATCAACGCAGAGTACTTTTTTTTT23950.14416767995377003No Hit
GTCCTACAGTGGACATTTCTAAATT23780.14314436030482888No Hit
GTCCTAAAGTGTGTATTTCTCATTT22040.1326703827215487No Hit
CTGTAGGACGTGGAATATGGCAAGA21260.12797515139111276No Hit
GTACATGGGAAGCAGTGGTATCAAC20380.12267796732600557No Hit
CTTTAGGACGTGAAATATGGCGAGG19480.1172603927139641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT1052.0147127E-812.65717411
TAGGACC5800.012.6041444
TCCAACG1103.842615E-812.0844118
GTATTAT2350.011.4617251
CCGGTTT1105.006859E-711.22055712
GGCGAGG5900.011.10554419
CCAACGA600.00589415911.07871919
TGGCGAG13800.010.9397718
GACGTGG6850.010.8113767
GGACCTG11050.010.65393456
ATGTAGG3650.010.5420951
GGACGTG14000.010.5112486
AGGACCT10850.010.500315
GTCCTAA7800.010.48296451
TGTAGGA16000.010.3853562
CGGTTTC1201.5268415E-610.28987613
GTATTAC1605.0222297E-910.2208911
GTCCATA1701.2551027E-910.1855241
AGGACGT14750.010.1698775
GTAGGAC15600.010.1647133
Produced by FastQC (version 0.11.5)