FastQCFastQC Report
Thu 26 May 2016
SRR1512790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661260
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52330.3150018660534775No Hit
GGTATCAACGCAGAGTACTTTTTTT33450.20135318974754102No Hit
TATCAACGCAGAGTACTTTTTTTTT32210.19388897583761722No Hit
CTGTAGGACGTGGAATATGGCAAGA24660.14844154436993606No Hit
GTCCTACAGTGGACATTTCTAAATT24270.1460939287047181No Hit
GTACATGGGAAGCAGTGGTATCAAC23270.14007440135800536No Hit
GTCCTAAAGTGTGTATTTCTCATTT22450.13513838893370092No Hit
CTTTAGGACGTGAAATATGGCGAGG22230.13381409291742413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4650.013.2910764
GACCGTC658.0346037E-411.6904557
AGGACGT14300.011.5036585
GGACGTG13900.011.41185956
GGCGAGG6500.011.24596519
TGTAGGA15050.010.9932372
GTAGGAC14000.010.9344653
CATTCGT700.001500237310.84953919
TAGGACG15450.010.7082984
CTGTAGG15450.010.4744461
GACGTGG7050.010.3742427
TGGCGAG15050.010.28183218
TAGGACT2500.010.2688884
AGACCGT750.002651218610.1317266
TTAGGAC9500.010.108753
GTTACAC1701.5352271E-910.0675383
CGTGGAA7550.010.064639
GTAACAC1554.0074156E-89.8149483
TATACTG3300.09.7969495
GACGTGA8350.09.782887
Produced by FastQC (version 0.11.5)