FastQCFastQC Report
Thu 26 May 2016
SRR1512789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059441
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52580.2553119997125433No Hit
GGTATCAACGCAGAGTACTTTTTTT31410.15251711508122837No Hit
TATCAACGCAGAGTACTTTTTTTTT27770.13484241597598573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA350.002180835716.27415312
CGTGCGC751.4925838E-512.65551110
TAGGACC3300.011.5106334
TCTACGC600.00589285411.0792553
GTGTACG700.001345376410.9923211
GTAGGAC8100.010.5516723
GGTATCA14200.010.4310941
TAGGACT3100.010.4152674
GGCGAGG4000.010.20599819
GTATAAG2750.010.1429161
GTCCTAC7300.010.0135551
CTAGGTT1901.0004442E-109.9960764
AGGACGT8400.09.9484765
TAGGACG8250.09.8991454
GACGTGG4250.09.8311957
TTATACT2450.09.6900744
GGACGTG7950.09.6752116
TATGGCG7850.09.56236716
TGTAGGA9300.09.4965052
TAGGTTG1502.6636735E-79.4962735
Produced by FastQC (version 0.11.5)