FastQCFastQC Report
Thu 26 May 2016
SRR1512789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059441
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66730.32401996464089045No Hit
GGTATCAACGCAGAGTACTTTTTTT41470.20136532194901433No Hit
TATCAACGCAGAGTACTTTTTTTTT38340.18616702299313262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG903.6019628E-813.733485
ACTGCGT802.8740315E-511.8723288
GGACCGT1302.6211637E-911.6908136
CGTGCGC752.0787783E-411.39743510
TAGGACC3550.010.7130074
GGTATCA14850.010.4478511
GTATCAA29350.010.2805651
ATAGGAC1409.575706E-810.1869213
GTCCTAG2450.010.1012031
GTCCTAC8450.010.025341
TAGGACA3650.09.8985254
GTTTAGG1951.5825208E-109.7625041
GTCTTAG2251.8189894E-129.7299621
TAGGACG8550.09.6745984
GTACTAC1502.575016E-79.5184411
TCCTACA9400.09.5094132
AGGACCG1606.5525455E-89.5077935
CCCGTGC800.0045248939.4978628
GGCGAGG3700.09.49394119
GTAGGAC9450.09.4574883
Produced by FastQC (version 0.11.5)