FastQCFastQC Report
Thu 26 May 2016
SRR1512788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293230
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49770.21703012781099149No Hit
GTCCTACAGTGGACATTTCTAAATT33460.14590773712187613No Hit
GTCCTAAAGTGTGTATTTCTCATTT30040.13099427445131975No Hit
GGTATCAACGCAGAGTACTTTTTTT29420.12829066425958147No Hit
CTGTAGGACGTGGAATATGGCAAGA28660.12497656144390228No Hit
TATCAACGCAGAGTACTTTTTTTTT25340.11049916493330368No Hit
CTTTAGGACGTGAAATATGGCGAGG24630.10740309519760337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6150.013.1258184
TCCAACG1501.4551915E-1112.02467518
ACCGCTC802.9923993E-511.8257168
GTGTAGG4900.011.3877341
CGTGCGC600.00592314211.07171410
GTATACG700.001342690110.9950541
AGCACCG1053.484185E-610.8529035
AATCCCG2450.010.85099819
GTCCTAC17700.010.7077541
GTAGGAC19300.010.5314153
GGCGAGG8500.010.49993619
TATACTG3350.010.48844055
AGGACGT18000.010.3931625
GGACGTG17650.010.3840466
TGTAGGA20950.010.3820162
TCCTACA20450.010.3571872
GGACCTG10950.010.2334456
GTCCTAA10900.010.1502511
TTAGGAC12150.010.0842523
TAGGACT4250.010.0555584
Produced by FastQC (version 0.11.5)