FastQCFastQC Report
Thu 26 May 2016
SRR1512788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293230
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62270.2715383978057151No Hit
GGTATCAACGCAGAGTACTTTTTTT37350.16287071074423412No Hit
TATCAACGCAGAGTACTTTTTTTTT36950.16112644610440296No Hit
GTCCTACAGTGGACATTTCTAAATT36230.1579867697527069No Hit
CTGTAGGACGTGGAATATGGCAAGA34080.14861134731361442No Hit
GTCCTAAAGTGTGTATTTCTCATTT31230.13618346175481744No Hit
CTTTAGGACGTGAAATATGGCGAGG29100.12689525254771652No Hit
GTACATGGGAAGCAGTGGTATCAAC24400.10640014302970045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG550.003049199712.1014735
TACACCG752.0578729E-411.4099615
TAGGACC5500.011.2363454
TCCAACG2400.011.07559918
TGGGCCG750.002629272210.1421875
TGGCGAG17100.010.04853718
TGTAGGA19400.010.0485292
CGGTTTC1803.965397E-1010.02449713
GTAGGAC18250.09.95050053
CGTGCGC1151.0289528E-59.91131310
CGAAATC1356.474438E-79.84862813
GTCCTAA10500.09.791731
GATATAC5250.09.791731
TCTTACA3350.09.6512692
GGCGAGG7750.09.55461819
CCTAATA1701.6389095E-89.5093382
CTGGACG800.00448683049.5076774
CCGTCTG800.00452333889.4983429
ACGAAAT1502.6559428E-79.49834212
AGGACGT18350.09.4823925
Produced by FastQC (version 0.11.5)