FastQCFastQC Report
Thu 26 May 2016
SRR1512787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011758
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39580.1967433458696324No Hit
GTACATGGGAAGCAGTGGTATCAAC24450.1215354928376077No Hit
GGTATCAACGCAGAGTACTTTTTTT23410.1163658849623066No Hit
TATCAACGCAGAGTACTTTTTTTTT20510.10195063223310159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4050.011.4871254
AGAGTCG752.0861322E-411.3930685
GTGTAGG3050.011.35164451
CGGGCGA600.00591431711.07382510
TGCACCG951.3698273E-510.9933115
AGGACGT8900.010.881025
GTCCTAA5700.010.629721
GTCTTAG2400.010.4188131
GGACGTG9150.010.2724386
GAATAAC1502.0207153E-810.2585231
TGGCGAG9450.010.14701218
GTAGGAC9750.010.1284373
CCTATAC1606.2227627E-910.0888733
TAGGTTG1054.133339E-59.9463295
TAGGACG9500.09.8942274
ACTAGAC1252.5862992E-69.8752263
GACGTGA5000.09.8742397
TCTACAC2700.09.8470933
CTACACT3000.09.8109434
TGTAGGA11300.09.7475012
Produced by FastQC (version 0.11.5)