FastQCFastQC Report
Thu 26 May 2016
SRR1512787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011758
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48150.23934290307283482No Hit
TATCAACGCAGAGTACTTTTTTTTT29470.1464887923895419No Hit
GGTATCAACGCAGAGTACTTTTTTT29400.1461408380133197No Hit
GTACATGGGAAGCAGTGGTATCAAC27770.13803847182414583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGA604.1021846E-412.66328510
CTAGTGC752.0585055E-411.409443
TAGGACC4200.011.3188894
GTCCTAG2400.011.1079961
TCTACAC3300.010.9484523
GTAGGAC10750.010.7018783
TGTAGGA10950.010.5079792
GTCCTAC9200.010.4525561
TGGCGAG10150.010.38303218
CCGCGCG750.00265402210.1306289
GTACTAA1605.9517333E-910.1162111
TAGGACA3600.010.0360814
GGCGAGG6250.010.0260819
GTCCTAA4950.09.8096591
AGGACGT11450.09.7987445
TAGGACT2950.09.6690174
ATGGCGA11200.09.66393817
GGACGTG11450.09.5389386
CCTTATA1401.044662E-69.5092872
TACACGT900.00110541199.5078674
Produced by FastQC (version 0.11.5)