FastQCFastQC Report
Thu 26 May 2016
SRR1512786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905149
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42240.2217149419809159No Hit
GTCCTACAGTGGACATTTCTAAATT26310.1380994347423745No Hit
GGTATCAACGCAGAGTACTTTTTTT25970.13631479742529326No Hit
TATCAACGCAGAGTACTTTTTTTTT23980.12586942018708247No Hit
CTGTAGGACGTGGAATATGGCAAGA22840.1198856362415748No Hit
GTCCTAAAGTGTGTATTTCTCATTT22600.11862589225304689No Hit
CTTTAGGACGTGAAATATGGCGAGG19600.10287909239644773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG250.00602799919.00248514
TAGACGT250.00604827118.9914424
CCGTGCG551.9986455E-413.786839
ACCGTGC957.793096E-812.9472918
AGGACGT14600.011.5769745
TGTAGGA16400.011.407352
GGACGTG14750.011.3954676
GTCGAGG752.0865466E-411.39275919
TAGGACC5450.011.3251714
TAGGACG15250.011.2080654
GTAGGAC15150.011.1575733
ATTAGGC600.005892723311.0792183
TCCAACG1701.4006218E-1010.60922218
GTCCTAC14150.010.6063371
GTCCTAT2100.010.5367281
TCCTACA15600.010.470472
TTAGGAC10100.010.4367193
TAAGACA2650.010.3915434
GGCGAGG7800.010.34598819
GTATAAG2053.6379788E-1210.32442951
Produced by FastQC (version 0.11.5)