FastQCFastQC Report
Thu 26 May 2016
SRR1512786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905149
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53720.2819726960988353No Hit
GGTATCAACGCAGAGTACTTTTTTT35160.18455249431934195No Hit
TATCAACGCAGAGTACTTTTTTTTT33430.17547183973536978No Hit
GTCCTACAGTGGACATTTCTAAATT27630.1450280266792781No Hit
CTGTAGGACGTGGAATATGGCAAGA26670.13998905072516638No Hit
GTCCTAAAGTGTGTATTTCTCATTT24710.1297011414855216No Hit
CTTTAGGACGTGAAATATGGCGAGG21860.11474168162175241No Hit
GTACATGGGAAGCAGTGGTATCAAC20650.10839047234625743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA250.006036998218.9975836
TCCAACG1155.3805707E-912.38289718
TAGGACC5250.012.3145174
GTCCTAA8450.011.602791
ATAGGAC1950.011.21371653
GACCGAG855.3324136E-511.1747567
CCAACGT855.359581E-511.16918219
TAAGACG600.00584191511.0921214
GACGTGG7600.010.623377
AGGACGT13900.010.6022035
TGTAGGA16300.010.5004962
TATACTG3100.010.427895
GTGTAGG2850.010.3537741
GGACGTG14100.010.30719956
GTAGGAC16700.010.247373
TGGCGAG13900.010.03994718
CTGTAGG16550.010.0076741
GTATTAA2400.09.915411
GGTATCA17200.09.9061861
CGTGGAA7700.09.8683569
Produced by FastQC (version 0.11.5)