FastQCFastQC Report
Thu 26 May 2016
SRR1512785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039046
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45220.22177037693117274No Hit
GTCCTACAGTGGACATTTCTAAATT30660.15036443513290038No Hit
GTCCTAAAGTGTGTATTTCTCATTT28650.14050688410168286No Hit
GGTATCAACGCAGAGTACTTTTTTT27230.13354284307465356No Hit
CTGTAGGACGTGGAATATGGCAAGA26250.12873667391515442No Hit
CTTTAGGACGTGAAATATGGCGAGG24890.12206688814278835No Hit
TATCAACGCAGAGTACTTTTTTTTT24820.12172359034568127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCCGT400.00530035414.2416716
TAGGACC6850.012.6139954
ATAGGAC2200.012.0850343
ATCGTGC600.006052491711.0395258
GGACTGT3200.010.9779546
TCTCGAA700.001499651910.85026112
GACGTGA9100.010.7461977
GGACGTG17000.010.667296
TGTAGGA19100.010.6388062
AGGACGT17250.010.5679935
TTAGGAC11600.010.5595223
GTAGGAC18300.010.2218073
TAGGACG18700.010.2060174
AAGACCG750.00266049510.127665
GGCGAGG9400.010.10047519
ACGTGAA11000.09.9785848
GTCCTAC16400.09.9747491
AGGACTG4000.09.9694155
AGGACCG1054.1312225E-59.9468085
CGTGAAA10950.09.8697699
Produced by FastQC (version 0.11.5)