FastQCFastQC Report
Thu 26 May 2016
SRR1512785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039046
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55630.2728236636152397No Hit
GGTATCAACGCAGAGTACTTTTTTT33890.16620517634226986No Hit
GTCCTACAGTGGACATTTCTAAATT33260.16311549616830617No Hit
TATCAACGCAGAGTACTTTTTTTTT32910.16139900718277075No Hit
GTCCTAAAGTGTGTATTTCTCATTT31540.1546801788679608No Hit
CTGTAGGACGTGGAATATGGCAAGA31180.15291464733998153No Hit
CTTTAGGACGTGAAATATGGCGAGG28910.14178199020522342No Hit
GTCCTACAGTGTGCATTTCTCATTT20730.1016651904861391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG350.002173865216.281959
AGGACCG751.4651514E-512.6783595
GCACCGA604.1018348E-412.6634286
TAGGACC6950.012.5862214
GTATTAC1751.8189894E-1211.4237141
GGTTAAC600.005786517611.1063881
AGCACCG600.00583649511.0935665
ACCGAGT600.005886841611.0807718
GGTATCA15450.010.9061351
GGCGAGG8100.010.78339619
TTAGGAC11700.010.7270533
ACCTTTT4700.010.50434315
GTAGGAC19100.010.5036793
TGTAGGA19750.010.448382
AGGACGT17750.010.4462545
GGACGTG17300.010.3759596
TAGGACA6400.010.2508884
GTATTAG1409.401083E-810.1997441
GTCCTAC16900.010.1957211
GTCCTAT2450.010.1026031
Produced by FastQC (version 0.11.5)