FastQCFastQC Report
Thu 26 May 2016
SRR1512784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943657
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46270.23805640604283576No Hit
GTCCTACAGTGGACATTTCTAAATT46060.23697596849649913No Hit
GTCCTAAAGTGTGTATTTCTCATTT40970.21078822034957817No Hit
CTGTAGGACGTGGAATATGGCAAGA37840.19468455596846562No Hit
CTTTAGGACGTGAAATATGGCGAGG33360.1716352216466177No Hit
GTACATGGGAAGCAGTGGTATCAAC31490.1620141825435249No Hit
GGTATCAACGCAGAGTACTTTTTTT28720.14776269681327517No Hit
TATCAACGCAGAGTACTTTTTTTTT25970.13361410989696226No Hit
GTCCTACAGTGTGCATTTCTCATTT24740.12728583283984776No Hit
CCCATGTACTCTGCGTTGATACCAC20510.1055227336922101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT350.00217657216.2788544
TAGGACC7850.013.4274644
CGTATCA654.838901E-513.3225631
GCGATCA550.00312246712.0630089
CCAACGT752.0824428E-411.39519819
GTGTAGG4050.011.1660991
AGCCCGT600.00589498711.0786666
AATGTCC6700.011.0102718
AGGACCT16000.010.9203985
GTCCTAG3350.010.9143381
GGACCTG15400.010.852576
TAGAAAT7400.010.7792424
TGTAGGA27500.010.6355182
AAATGTC6800.010.6131747
CCAGTAC2001.8189894E-1210.4455983
TGTCCAC8050.010.37346510
GGCGAGG11250.010.29788319
CTTAGAC1951.4551915E-1110.22645953
TTAGGAC15450.010.202823
TAGGACA8200.010.1908274
Produced by FastQC (version 0.11.5)