FastQCFastQC Report
Thu 26 May 2016
SRR1512784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943657
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59150.3043232422181486No Hit
GTCCTACAGTGGACATTTCTAAATT49310.25369702576123254No Hit
CTGTAGGACGTGGAATATGGCAAGA44940.23121363491603714No Hit
GTCCTAAAGTGTGTATTTCTCATTT43210.2223128875105021No Hit
CTTTAGGACGTGAAATATGGCGAGG39980.2056947290597055No Hit
GGTATCAACGCAGAGTACTTTTTTT37340.1921120856200451No Hit
TATCAACGCAGAGTACTTTTTTTTT36110.1857838085629306No Hit
GTACATGGGAAGCAGTGGTATCAAC33760.1736931979253541No Hit
GTCCTACAGTGTGCATTTCTCATTT27370.14081702687253975No Hit
CCCATGTACTCTGCGTTGATACCAC23060.1186423324691548No Hit
CTGTAGGACCTGGAATATGGCGAGA21520.11071912379601956No Hit
CTGAAGGACCTGGAATATGGCGAGA20640.10619157598279945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG400.005286283314.2473367
CCAACGA957.453491E-812.9915519
TAGGACC8500.012.5288524
TCGAACT1103.850255E-812.08305119
TCCAACG1501.7826096E-1011.392590518
ATTGCGA752.0868878E-411.392590517
TGTAGGA25550.011.1273682
ACCTTTT6500.010.95497815
CTCGAAC1303.2985554E-810.95441318
CTGTAGG25300.010.9094761
GTAGGAC25400.010.8184523
CGGTTTC1158.860716E-710.73518913
GTCCTAC24300.010.5750871
GACGTGG12300.010.3476587
CACCTTT7250.010.34579414
TAGGACT2900.010.1642624
AGGACCT17150.010.14615
CCCTTAT856.483389E-410.0774351
TAAGACC1701.5352271E-910.0678274
TCCTACA26450.010.0297642
Produced by FastQC (version 0.11.5)