FastQCFastQC Report
Thu 26 May 2016
SRR1512783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073829
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54210.26140053013049774No Hit
CTGTAGGACGTGGAATATGGCAAGA48300.23290251992811364No Hit
GTATCAACGCAGAGTACTTTTTTTT45000.21698992539886364No Hit
GTCCTAAAGTGTGTATTTCTCATTT44370.21395206644327955No Hit
CTTTAGGACGTGAAATATGGCGAGG38260.18448965657245606No Hit
GTCCTACAGTGTGCATTTCTCATTT25370.12233409794153713No Hit
GGTATCAACGCAGAGTACTTTTTTT25340.12218943799127123No Hit
TATCAACGCAGAGTACTTTTTTTTT23410.11288298119083105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1404.1836756E-1112.3703451
TAGGACC8450.011.7994724
ACAGCGC1157.4869604E-811.5184148
TCCAACG2250.011.39602518
TATGTCG1354.7093636E-911.2624416
CCAACGA1451.2223609E-911.13539819
CTTAGAC1553.1468517E-1011.0276083
ATCCCGT1658.185452E-1110.92603410
CTCGAAC1252.2516906E-710.6362918
GGCGAGG11550.010.52574419
TAGGACT3700.010.5223354
TTTAGAA7950.010.3918442
TAGACTG2750.010.3590165
GTTTAGG3100.010.2421131
GTGTAGG4800.010.2227161
GAAATGT7250.010.2161336
TGTAGGA29200.010.2114482
GTCCTAC25150.010.1378351
TATTGCG750.002641999210.13619616
GATATAC4850.010.1173281
Produced by FastQC (version 0.11.5)