FastQCFastQC Report
Thu 26 May 2016
SRR1512783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073829
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57950.2794348039303144No Hit
GTCCTACAGTGGACATTTCTAAATT57610.27779532449396743No Hit
CTGTAGGACGTGGAATATGGCAAGA56780.27379306586994395No Hit
GTCCTAAAGTGTGTATTTCTCATTT48120.2320345602265182No Hit
CTTTAGGACGTGAAATATGGCGAGG45210.218002545050725No Hit
GGTATCAACGCAGAGTACTTTTTTT35280.1701201015127091No Hit
TATCAACGCAGAGTACTTTTTTTTT33190.16004212497751744No Hit
GTCCTACAGTGTGCATTTCTCATTT28380.13684831295155No Hit
CTGAAGGACCTGGAATATGGCGAGA25380.1223823179249591No Hit
CTGTAGGACCTGGAATATGGCGAGA20850.10053866543480681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7850.013.3212384
GGCGAGG12800.012.38808219
CTAACAC1950.012.1878453
TGTAGGA29250.011.2474922
TGTAGAA6450.011.2036452
AGGACGT30750.011.1613085
GGACCTG17150.011.1318076
TTAGGAC17450.011.1136393
GGACGTG29700.011.065056
CGGTTTC1553.1468517E-1011.02891113
AGGACCT18100.010.9779365
GTCTTAT2100.010.8779891
TATACTG3150.010.865385
GACGTGA16100.010.8551587
CACCTTT7000.010.85201614
GTAGGAC29300.010.7070023
CTGTAGG28700.010.6790021
GACCGTG909.5353316E-510.5536267
TAGGACG34000.010.5130924
ACCTTTT7500.010.50786215
Produced by FastQC (version 0.11.5)