FastQCFastQC Report
Thu 26 May 2016
SRR1512782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555052
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45420.2920802648400182No Hit
GGTATCAACGCAGAGTACTTTTTTT28730.1847526642195888No Hit
TATCAACGCAGAGTACTTTTTTTTT25170.1618595391022294No Hit
GTCCTACAGTGGACATTTCTAAATT20390.13112101717498836No Hit
GTCCTAAAGTGTGTATTTCTCATTT19510.12546204242687703No Hit
CTGTAGGACGTGGAATATGGCAAGA18430.11851693705419498No Hit
CTTTAGGACGTGAAATATGGCGAGG16250.10449811324637376No Hit
GTACATGGGAAGCAGTGGTATCAAC15810.1016686258723181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTAC1457.8216544E-1111.9425911
TAGGACC4300.011.925784
ATGGGCG658.0507156E-411.6875665
CTAGGAC2150.011.4848293
GTCTAAT1052.9798357E-610.9947661
TTAGGAC7300.010.9281353
GTATTAG2050.010.7936421
TAGGACT1701.4006218E-1010.6136854
GGACCTG8600.010.6006946
TAGACAG2150.010.6003495
TGGCGAG13000.010.36935818
TAGGACA4000.010.2086884
AGGACGT11550.010.1949995
GGCGAGG6800.010.19208419
TTAGACT1502.4574547E-810.1295524
CTATTGG1053.5733705E-510.0785361
GACGTGA7450.010.07006557
TCTAGGA2850.09.9969162
GTCCTAC11200.09.878111
GGACGTG11650.09.8632716
Produced by FastQC (version 0.11.5)