FastQCFastQC Report
Thu 26 May 2016
SRR1512782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555052
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54130.34809125354007453No Hit
GGTATCAACGCAGAGTACTTTTTTT35630.2291241707672798No Hit
TATCAACGCAGAGTACTTTTTTTTT33300.21414074899103053No Hit
CTGTAGGACGTGGAATATGGCAAGA21990.1414100621715544No Hit
GTCCTAAAGTGTGTATTTCTCATTT21210.13639415273572847No Hit
GTCCTACAGTGGACATTTCTAAATT20750.1334360522992157No Hit
CTTTAGGACGTGAAATATGGCGAGG19010.12224671586545015No Hit
GTACATGGGAAGCAGTGGTATCAAC18420.11845263052296645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG500.001501922313.2972087
TGCACCG655.407427E-513.1654845
GGTTAGA604.0165792E-412.6946361
GGCGAGG5200.011.50164919
GTGCTAT951.3272296E-511.024291
TAGGACC4250.010.96192554
TAGGACT2750.010.3721384
TTCCACC4600.010.32391911
GTAATAC1303.8002509E-710.23850253
AGCCCGT856.605685E-410.0567116
GAGGGCG856.605685E-410.0567119
AGGACGT11300.010.0132775
GGACGTG11500.09.9935536
CTACACT2105.456968E-129.96054654
GTAGGAC11300.09.9278533
TGGCGAG12450.09.91281218
TGTAGGA12000.09.9058662
GTCCTAC13700.09.8684591
GACGTGA7100.09.6317817
CCACCTT4150.09.61120213
Produced by FastQC (version 0.11.5)