FastQCFastQC Report
Thu 26 May 2016
SRR1512781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237387
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40210.3249589659500221No Hit
GTCCTACAGTGGACATTTCTAAATT33520.2708934229953927No Hit
GTCCTAAAGTGTGTATTTCTCATTT31820.2571547947408531No Hit
CTGTAGGACGTGGAATATGGCAAGA30610.24737612404203374No Hit
CTTTAGGACGTGAAATATGGCGAGG27550.22264659318386246No Hit
GGTATCAACGCAGAGTACTTTTTTT24630.19904847877018264No Hit
TATCAACGCAGAGTACTTTTTTTTT23320.18846165346815508No Hit
GTACATGGGAAGCAGTGGTATCAAC18420.14886207791095268No Hit
GTCCTACAGTGTGCATTTCTCATTT15100.12203134508444004No Hit
CTGAAGGACCTGGAATATGGCGAGA13660.11039391879824179No Hit
GTCCTTCAGTGTGCATTTCTCATTT12550.10142340270263063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4950.012.4695424
GTATTAG1801.8189894E-1211.2229361
AATCCCG855.3609372E-511.16817219
ACCGTGC600.00605884211.0374118
TGTCGAG951.37315365E-510.99004618
ACCTTTT4700.010.916553515
GTGTAGG1501.7316779E-910.9022821
CACCTTT4200.010.85879314
AGGACGA700.001496727910.852175
CTACACT1803.6379788E-1110.5511494
ATGTAGG2200.010.4941751
TCCTATA1553.6779966E-910.4154292
TGTAGGA16200.010.375722
AGGACCT10050.010.2987635
GCTCTAG750.002385687810.2609711
TCCTACA18100.010.1784842
GGCGAGG8400.010.17101419
GTCCTAC16850.010.1049241
CCTACAG18050.010.0488443
CTTGGAC951.6494472E-49.9962333
Produced by FastQC (version 0.11.5)