FastQCFastQC Report
Thu 26 May 2016
SRR1512781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237387
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50190.40561279535020167No Hit
GTCCTACAGTGGACATTTCTAAATT36500.29497643017099745No Hit
GTCCTAAAGTGTGTATTTCTCATTT34100.27558071969400033No Hit
CTGTAGGACGTGGAATATGGCAAGA33610.2716207621382801No Hit
CTTTAGGACGTGAAATATGGCGAGG32450.2622461687410648No Hit
GGTATCAACGCAGAGTACTTTTTTT31520.2547303309312285No Hit
TATCAACGCAGAGTACTTTTTTTTT30260.244547582930805No Hit
GTACATGGGAAGCAGTGGTATCAAC19450.15718607032399726No Hit
GTCCTACAGTGTGCATTTCTCATTT16810.13585078879930046No Hit
CTGAAGGACCTGGAATATGGCGAGA16620.13431529505320486No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14610.11807138752871979No Hit
CCCATGTACTCTGCGTTGATACCAC14570.1177481256874365No Hit
CTGTAGGACCTGGAATATGGCGAGA13950.11273756714754558No Hit
GTCCTTCAGTGTGCATTTCTCATTT13130.10611069940123827No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12620.10198911092487636No Hit
TTTCTAAATTTTCCACCTTTTTCAG12430.10045361717878076No Hit
ATTTAGAAATGTCCACTGTAGGACG12390.10013035533749748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4600.013.2258354
TGTCGAG701.0951769E-412.20875218
TCCAACG802.8785698E-511.8696218
GCGCCAC658.043755E-411.68842813
TTAGGAC10900.011.4247253
GTGTAGG1501.70985E-1011.4216091
CCAACGA752.0819061E-411.39483519
GATATAC1803.6379788E-1211.10434151
TAGGACA4550.011.0730034
AGGACCT11950.010.8994945
GTCATAC700.001467656310.8777221
GGTCTAC1158.596835E-710.7594861
GGCGAGG8300.010.75416219
CACCTTT4150.010.75329314
GACGTGA9550.010.6417377
TGGCGAG18050.010.62676218
GGACCTG11300.010.5070616
TAGAGGA1553.61797E-910.4260114
GTATTAA1658.967618E-1010.383281
GTCCTAA9200.010.345661
Produced by FastQC (version 0.11.5)