FastQCFastQC Report
Thu 26 May 2016
SRR1512780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594658
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35280.22123866057800484No Hit
GTCCTACAGTGGACATTTCTAAATT26210.1643612611607003No Hit
GTACATGGGAAGCAGTGGTATCAAC25880.16229185192059992No Hit
GTCCTAAAGTGTGTATTTCTCATTT24100.15112958389824027No Hit
CTGTAGGACGTGGAATATGGCAAGA23060.14460780932337844No Hit
GGTATCAACGCAGAGTACTTTTTTT21930.13752165041030742No Hit
CTTTAGGACGTGAAATATGGCGAGG19600.12291036698778045No Hit
TATCAACGCAGAGTACTTTTTTTTT19300.12102908586041647No Hit
CCCATGTACTCTGCGTTGATACCAC17740.11124642399812375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGCA456.8891456E-414.7416369
CGTATCA500.001364373713.4677351
ACAAGCG500.00150587313.2925985
ACACCGT751.4871113E-512.6596186
GATCTAG709.70421E-512.3683291
CGGAGAC600.00589321211.0789133
CTAACAC1408.400093E-910.8528133
TAGGACC3650.010.665634
GTATTAG1451.1868906E-810.61496451
GGCGAGG5850.010.55034619
GGACGTG13050.010.4769256
TGTAGGA14450.010.4491192
AGGACGT13500.010.40901855
ACCGTGC1106.3605257E-610.3194338
TTTAGGA8850.010.1936742
CTTATAC1502.458546E-810.12929253
TCTATAC1502.458546E-810.12929253
GAACAGT3050.09.9616676
TAGGACG14700.09.9471564
TATACTG2201.8189894E-129.92629055
Produced by FastQC (version 0.11.5)