FastQCFastQC Report
Thu 26 May 2016
SRR1512780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594658
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44690.2802481786063219No Hit
GTACATGGGAAGCAGTGGTATCAAC28940.18148091941971256No Hit
GGTATCAACGCAGAGTACTTTTTTT28500.1787217070995787No Hit
GTCCTACAGTGGACATTTCTAAATT28290.17740481031042393No Hit
CTGTAGGACGTGGAATATGGCAAGA26590.16674421725536134No Hit
TATCAACGCAGAGTACTTTTTTTTT25730.1613512113569179No Hit
GTCCTAAAGTGTGTATTTCTCATTT25610.16059869890597234No Hit
CTTTAGGACGTGAAATATGGCGAGG23670.14843308094901855No Hit
CCCATGTACTCTGCGTTGATACCAC20700.12980839778811507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC307.7353773E-418.995427
CGCACTT350.002174185616.28127712
TAGGACC4150.012.83107954
AATCCCG951.0475997E-611.99259219
ATACGCA752.0562261E-411.4104964
ACAGCGC1001.9350937E-611.3972538
GACGTGG7250.011.3972527
ATTAGAC1158.711304E-710.7490183
CGTGGAA7550.010.5669899
GTAGGAC15250.010.5375623
TTAGTAC1105.9783433E-610.37317853
ACGTGGA7800.010.3500688
GTACTAG750.002606347210.1525481
TGTAGGA15950.010.1353512
GTCCTAC13100.010.0992661
GGACGTG14200.010.03342156
AGGACGT14900.010.0195995
TGACGAA1356.494847E-79.84577118
TCCTACA14300.09.8418392
GTCCTAA8150.09.8099781
Produced by FastQC (version 0.11.5)