FastQCFastQC Report
Thu 26 May 2016
SRR1512779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497826
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37000.24702468778082368No Hit
GGTATCAACGCAGAGTACTTTTTTT22930.1530885429949807No Hit
GTCCTACAGTGGACATTTCTAAATT20380.13606386856684285No Hit
TATCAACGCAGAGTACTTTTTTTTT19710.13159071881513607No Hit
GTCCTAAAGTGTGTATTTCTCATTT19660.1312569016694863No Hit
CTGTAGGACGTGGAATATGGCAAGA19290.12878665479167806No Hit
CTTTAGGACGTGAAATATGGCGAGG15020.10027867055318843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC853.405632E-612.4562021
TAGGACC3350.011.0542284
TTAGGAC8450.010.5638823
TAGAAAT3800.010.4948034
AGGACGT13600.010.4731075
AGCACCG1002.4104293E-510.4451795
TTATACC1002.4113227E-510.4448284
ATTACAC1752.3646862E-1010.310223
GGACGTG13450.010.2369116
GTCTTAG1604.973117E-910.2268251
GTGTAGA2550.010.1914381
GGCGAGG5600.010.17216819
GTCTTAT951.4400935E-410.1318391
TAGGCCC750.00265835410.1283184
GTATAAA2105.456968E-1210.0835921
GGTATCA11750.010.075791
GACGTGA7200.09.8912697
GTTCTAA1855.11136E-109.8853881
GTAGCAC1651.027729E-89.7840193
TTACTCT1752.59206E-99.7665934
Produced by FastQC (version 0.11.5)